上皮性卵巢癌转移模式相关分子初探

发布时间：2018-05-10 15:16

本文选题：卵巢癌 + 上皮性　；参考：《现代妇产科进展》2017年12期

【摘要】：目的:筛选和比较不同临床分型(淋巴结转移型/腹膜转移型/混合型)的III期卵巢癌原发灶中的差异表达分子,为晚期卵巢癌的分子分型提供参考。方法:用Tumor Metastasis PCR Array检测淋巴结转移型与腹膜转移型肿瘤组织中肿瘤转移相关分子mRNA表达,Western blot、免疫组化法在肿瘤组织及石蜡包埋组织切片中进一步验证PCR array法筛选出的差异表达分子在蛋白水平的差异表达情况。结果:Tumor Metastasis PCR array筛选出差异表达分子共14个,挑选文献报道的与其它肿瘤淋巴结转移和(或)腹膜转移相关的SMAD4、CCL7、CDKN2A及SERPINE1 4个分子。Western blot结果示,p16(CDKN2A)、CCL7及PAI-1(SERPINE1)在淋巴结转移型原发灶组织中的蛋白表达水平均显著高于腹膜转移型组织。免疫组化结果显示,72例III期上皮性卵巢癌的石蜡包埋组织中,PAI-1蛋白表达水平在淋巴结转移组、腹膜转移组及混合组之间有显著差异,平均评分分别为10.7、7.28及8.13分(P=0.036)。结论:PAI-1表达水平在不同转移模式的IIIc期卵巢癌中存在显著差异,在淋巴结转移型组织中最高,其次为混合型,腹膜转移型最低,可考虑作为卵巢癌分型的潜在分子标记物。
[Abstract]:Objective: to screen and compare the differentially expressed molecules in the primary foci of III stage ovarian cancer with different clinical types (lymph node metastasis / peritoneal metastasis / mixed type), and to provide a reference for the molecular typing of advanced ovarian cancer. Methods: Tumor Metastasis PCR Array was used to detect the mRNA expression of tumor metastasis related molecules in lymph node metastasis and peritoneal metastasis. Immunohistochemistry was used to further verify the screening of tumor tissues and paraffin embedded tissues by PCR array method. The differential expression of the expressed molecules at the protein level was different. Results 14 differentially expressed molecules were screened by Metastasis PCR array. The results of SMAD4 CCL7CDKN2A and SERPINE1 4 molecules. Western blot results showed that the protein expression levels of p16 + CDKN2A CCL7 and PAI-1 serpINE1) in the primary lymph node metastasis tissues were significantly higher than those in the peritoneal metastatic tissues, which were associated with other lymph node metastases and / or peritoneal metastases in the literature. The results showed that the expression of CDKN7 and PAI-1SERPINE1 in the primary lymph node metastasis tissues were significantly higher than those in the peritoneal metastatic tissues. Immunohistochemical results showed that the expression of PAI-1 protein in paraffin embedded tissues of 72 cases of III epithelial ovarian carcinoma was significantly different between lymph node metastasis group, peritoneal metastasis group and mixed group. The average scores were 10.7 / 7.28 and 8.13 respectively. Conclusion there are significant differences in the expression level of 1% PAI-1 in ovarian carcinoma of IIIc stage with different metastasis patterns, which is the highest in lymph node metastatic tissues, followed by mixed type, and lowest in peritoneal metastasis type. It may be considered as a potential molecular marker for ovarian cancer classification.
【作者单位】：华南肿瘤学国家重点实验室中山大学肿瘤防治中心妇科;
【基金】：国家高技术研究发展计划(863计划)(No:2012AA02A507)
【分类号】：R737.31

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