7个单核甘酸多态性与女性乳腺癌的关联性研究

发布时间：2018-05-10 20:19

本文选题：乳腺癌 + 危险因素　；参考：《宁夏医科大学》2015年硕士论文

【摘要】：目的:调查分析乳腺癌的环境危险因素,研究rs6788895(SIAH2基因)、rs10941679(5p12)、rs889312(MAP3K1)、rs13387042(2q35)、rs6504950(17q23)、rs17530068(6q14)和rs2284378(20q11)7个位点的单核苷酸多态性与宁夏女性乳腺癌的关系,对环境因素和以上7个位点进行交互作用分析,探讨基因-环境交互作用对乳腺癌的影响。方法:采用以医院为基础且以年龄为频数匹配条件的病例-对照研究方法,共收集病例组与对照组研究对象各225人,其中病例组来源于2013年5月至2014年7月于宁夏医科大学附属总医院就诊入院的新发宁夏女性乳腺癌患者;对照组来源于同一医院同期入院的无乳腺癌的女性患者。研究对象使用统一的调查表进行流行病学调查,调查内容主要包括一般人口学资料、月经史、生育史、生活习惯和肿瘤家族史等其他乳腺癌易感环境因素。同时采集研究对象的静脉血2ml,采用Sequenom Mass Array系统进行基因型检测,所有统计计算均由SPSS17.0统计软件完成。结果:1.婚姻状况、文化程度、年龄、民族、职业及熬夜情况在病例组与对照组之间的分布差异均无统计学意义(P0.05)。2.病例组与对照组研究对象在初婚年龄、月经持续天数、首次生育年龄、生育次数、流产次数、是否绝经、月经规律性以及乳腺自检方面的分布差异具有统计学意义(P0.05);两组研究对象在初潮年龄、月经周期、末次生育年龄、绝经年龄、哺乳年限、行经年限、是否避孕、医院乳腺检查、是否哺乳、及BMI方面的分布差异均无统计学意义(P0.05)。3.体育锻炼及晚上佩戴文胸情况在病例组与对照组之间的分布差异有统计学意义(P0.05);而吸烟、被动吸烟、饮酒、失眠情况、午睡情况和饮食习惯方面在两组中分布差异均无统计学意义(P0.05)。4.癌症家族史在病例组与对照组间的分布差异具有统计学意义(P0.05),且病例组中有癌症家族史人群所占比例(9.8%)显著高于对照组(1.8%)。5.经logistic回归分析发现流产次数(OR=1.565)和癌症家族史(OR=5.030)为乳腺癌的危险性因素,乳腺自检(OR=0.418)、体育锻炼(OR=0.444)和晚上不佩戴文胸(OR=0.562)为可能会降低患乳腺癌的保护性因素。6.rs6788895、rs10941679、rs889312、rs13387042、rs6504950、rs17530068和rs2284378位点的基因型频率及等位基因频率在病例组与对照组间的分布差异均无统计学意义。7.rs6788895、rs889312、rs13387042、rs6504950、rs17530068、rs10941679和rs2284378位点多态性与环境因素(流产次数、癌症家族史、乳腺自检、体育锻炼、晚上是否佩戴文胸)之间不存在交互效应(P0.05)。结论:1.流产次数、乳腺自检、体育锻炼、晚上佩戴文胸和癌症家族史可能是女性乳腺癌的环境影响因素。2.rs6788895、rs10941679、rs889312、rs13387042、rs6504950、rs17530068和rs2284378位点的多态性与乳腺癌的发生可能不相关。3.s6788895、rs10941679、rs889312、rs13387042、rs6504950、rs17530068和rs2284378位点和环境因素可能不存在交互效应。
[Abstract]:Objective: To investigate and analyze the environmental risk factors of breast cancer, and to study the relationship between single nucleotide polymorphism of rs6788895 (SIAH2 gene), rs10941679 (5p12), rs889312 (MAP3K1), rs6504950 (2q35), rs6504950 (17q23), rs17530068 (6q14) and 7 loci with female breast cancer in Ningxia, and the interaction between the environmental factors and the above 7 loci. The effect of gene environmental interaction on breast cancer was analyzed. Methods: a case-control study based on the hospital based and matched condition of age was used to collect 225 people from the case group and the control group. The case group came from May 2013 to July 2014 at the General Hospital of Ningxia Medical University. A new Ningxia female breast cancer patient was admitted to the hospital. The control group was derived from women who had no breast cancer in the same hospital during the same period. The subjects used a unified questionnaire for epidemiological investigation, including general demographic data, menstrual history, birth history, life habits and family history of cancer. At the same time, the venous blood 2ml of the subjects was collected and the Sequenom Mass Array system was used for genotyping. All the statistical calculations were completed by the SPSS17.0 software. Results: there was no significant difference in the distribution difference between the case group and the control group in 1. marital status, cultural degree, age, nationality, occupation and staying up. (P0.05 The.2. case group and the control group were studied at the age of first marriage, the duration of menstruation, the first birth age, the number of birth, the number of abortion, the menopause, the regularity of menstruation and the difference in the distribution of breast self examination (P0.05); the two groups of subjects were at the age of menarche, the menstrual cycle, the last birth age, the menopause age, lactation. Years, menstruation, contraception, hospital breast examination, breast feeding, and BMI distribution were not statistically significant (P0.05).3. physical exercise and wearing a bra at night between the case group and the control group had a statistically significant difference (P0.05), while smoking, passive smoking, drinking, insomnia, nap and diet The distribution difference between the two groups was not statistically significant (P0.05).4. cancer family history between the case group and the control group had statistical significance (P0.05), and the proportion of the family history of cancer (9.8%) in the case group was significantly higher than the control group (1.8%).5. by logistic regression analysis to find the frequency of abortion (OR=1.565) and cancer. Family history of the disease (OR=5.030) is a risk factor for breast cancer, breast self examination (OR=0.418), physical exercise (OR=0.444) and not wearing a bra at night (OR=0.562) may reduce the protective factors of breast cancer, the genotype frequency and allele frequency of.6.rs6788895, rs10941679, rs889312, rs13387042, rs6504950, rs17530068, and rs2284378 loci. There was no significant difference in the distribution of.7.rs6788895, rs889312, rs13387042, rs6504950, rs17530068, rs10941679, and rs2284378 locus polymorphism and environmental factors (abortion times, cancer family history, breast self examination, physical exercise, and whether to wear a bra at night). There was no interaction effect (P0.05). Conclusion: 1. The number of miscarriages, self examination of breast, physical exercise, the history of wearing a bra and a family history of cancer at night may be the environmental factors of female breast cancer.2.rs6788895, rs10941679, rs889312, rs13387042, rs6504950, rs17530068 and rs2284378 polymorphism and breast cancer can not be associated with.3.s6788895, rs10941679, rs889312, rs13387042, rs6504 950, there may be no interaction effect between rs17530068 and rs2284378 loci and environmental factors.

【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R737.9

【引证文献】