结直肠癌组织中RPS15a的表达以及沉默RPS 15a对结肠癌RKO细胞增殖及凋亡的影响

发布时间：2018-05-11 13:17

本文选题：结直肠肿瘤 + 核糖体蛋白质类　；参考：《肿瘤》2017年07期

【摘要】：目的 :探讨核糖体蛋白S15a(ribosomal protein S15a,RPS15a)在结直肠癌中的作用。方法:采用免疫组织化学法检测RPS15a在120例结直肠癌及癌旁组织和120例结直肠腺瘤组织中的表达。将重组慢病毒液RPS15a-si RNA感染结肠癌RKO细胞后,采用Cellomics细胞计数实验、克隆形成实验和FCM法分别检测RKO细胞的增殖能力、细胞周期分布和细胞凋亡情况。结果:结直肠癌组织中RPS15a的阳性率明显高于结直肠腺瘤组织(86.7%vs25.8%,P0.001)和结直肠癌旁组织(86.7%vs 11.7%,P0.001),并且RPS15a的表达与结直肠癌患者的TNM分期(P=0.033)及肿瘤分化程度(P0.001)明显相关。沉默RPS15a表达可明显抑制RKO细胞增殖(P0.01)及克隆形成(P0.01),并诱导细胞凋亡(P0.01),且阻滞细胞周期于G2/M期(P0.01)。结论:结直肠癌组织中RPS15a的阳性率明显高于结直肠腺瘤组织和结直肠癌旁组织。下调RPS15a基因表达可抑制RKO细胞的增殖并诱导其凋亡。
[Abstract]:Objective: to investigate the role of ribosomal protein S15a(ribosomal protein S 15 a in colorectal cancer. Methods: immunohistochemical method was used to detect the expression of RPS15a in 120 cases of colorectal carcinoma and adjacent tissues and 120 cases of colorectal adenoma. After RKO cells were infected with recombinant lentivirus RPS15a-si RNA, the proliferation, cell cycle distribution and apoptosis of RKO cells were detected by Cellomics cell count assay, clone formation assay and FCM assay respectively. Results: the positive rate of RPS15a in colorectal cancer tissues was significantly higher than that in colorectal adenoma tissues (86.7 vs 25.8) and adjacent colorectal cancer tissues (86.7 vs 11.7T P 0.001), and the expression of RPS15a was significantly correlated with TNM staging and tumor differentiation (P0.001). Silencing the expression of RPS15a could significantly inhibit the proliferation of RKO cells (P0.01) and the formation of P0.01a, induce apoptosis and block the cell cycle at G _ 2 / M phase (P _ (0.01). Conclusion: the positive rate of RPS15a in colorectal carcinoma is higher than that in colorectal adenoma and paracarcinoma. Down-regulation of RPS15a gene expression can inhibit the proliferation and induce apoptosis of RKO cells.
【作者单位】：上海交通大学医学院附属仁济医院胃肠外科;
【分类号】：R735.34

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