维生素C碳点对口腔鳞状细胞癌KB细胞增殖、自噬和凋亡的影响

发布时间：2018-05-15 02:05

  本文选题：维生素C碳点 + 口腔黏膜　； 参考：《吉林大学学报(医学版)》2017年04期

【摘要】：目的:研究维生素C碳点对口腔黏膜鳞状细胞癌(口腔鳞癌)KB细胞的杀伤作用,探讨其相关作用机制。方法:以不同浓度(5、10、20、40和80mg·L~(-1))维生素C碳点体外处理口腔鳞癌KB细胞作为实验组,以0mg·L~(-1)维生素C碳点组作为空白对照组。MTT法检测各组细胞增殖率,克隆形成实验检测各组细胞克隆形成能力,Western blotting法检测各组细胞中自噬相关蛋白LC3蛋白表达水平,流式细胞术检测KB细胞的凋亡率。结果:与空白对照组比较,20、40和80mg·L~(-1)维生素C碳点组KB细胞的增殖率及克隆形成能力均明显降低(P0.01),40mg·L~(-1)维生素C碳点组KB细胞中LC3Ⅱ蛋白表达水平和细胞凋亡率明显升高(P0.05或P0.01)。结论:维生素C碳点能够有效地杀伤口腔鳞癌KB细胞,抑制KB细胞的增殖并减弱其克隆形成能力,其杀伤作用可能与KB细胞自噬和凋亡的发生有关。
[Abstract]:Aim: to study the killing effect of vitamin C carbon spot on KB cells in oral squamous cell carcinoma (OSCC) and its related mechanism. Methods: KB cells of oral squamous cell carcinoma were treated with Vitamin C carbon spots at different concentrations of 5, 10, 20, 40 and 80mg, respectively. The proliferation rate of KB cells was measured by 0mg LX-1) Vitamin C carbon spot group as a blank control group. The proliferation rate of KB cells in each group was measured by MTT assay. The expression of autophagy associated protein (LC3) protein was detected by Western blotting assay and the apoptosis rate of KB cells was detected by flow cytometry. Results: compared with the control group, the proliferation rate and clone formation ability of KB cells in the vitamin C carbon spot group were significantly lower than those in the control group. The expression level of LC3 鈪，

本文编号：1890469