SIRT1和突变型p53在结肠癌组织中的表达意义及相关性研究

发布时间：2018-05-16 07:16

本文选题：结肠癌 + SIRT1　；参考：《延边大学》2017年硕士论文

【摘要】：目的:探讨SIRT1(silent information regulator 1,沉默信息调节因子1)和突变型p53在结肠癌组织及癌旁组织中的表达意义,分析两种蛋白的表达与结肠癌患者临床病理特征的关系,并对SIRT1、突变型p53在结肠癌组织中的表达进行相关性分析。方法:1.收集病理诊断为结肠癌的癌组织和癌旁组织石蜡包埋组织标本各58例,常规进行HE染色,采用免疫组织化学方法检测SIRT1、突变型p53在结肠癌组织及癌旁组织中的表达。2.分析SIRT1和突变型p53在结肠癌组织中的表达与临床病理特征的关系。3.分析SIRT1与突变型p53在结肠癌组织表达的相关性。结果:1.SIRT1在结肠癌组织中主要定位于细胞核,少量定位于胞浆,在癌组织中的阳性表达率为43.1%(25/58),在癌旁组织中的阳性表达率为7%(4/58),两者之间差异有统计学意义(P0.05)。2.突变型p53在结肠癌组织中全部定位于细胞核,在癌组织中的阳性表达率为51.7%(30/58),在癌旁组织中阳性表达率为0%(0/58),两者之间差异有统计学意义(P0.05)。3.SIRT1在结肠癌中的表达与年龄、分化程度有关(P0.05),与性别、民族、肿瘤大小、浸润深度、淋巴结转移、远处转移、TNM分期均无关(P0.05)。4.突变型p53在结肠癌中的表达与年龄有关(P0.05),与性别、民族、肿瘤大小、分化程度、浸润深度、远处转移、TNM分期均无关(P0.05)。5.SIRT1在结肠癌组织中的表达与突变型p53在结肠癌组织中的表达呈正相关,相关系数为0.417,P=0.0010.05。结论:1.SIRT1在结肠癌组织中高表达,与患者年龄、分化程度有关。2.p53在结肠癌组织中高表达,与年龄有关。3.结肠癌中SIRT1与突变型p53呈正相关,SIRT1与p53可能起协同作用促进结肠癌的发生。
[Abstract]:Objective: to investigate the expression significance of SIRT1(silent information regulator 1 (silencing signal regulator 1) and mutant p53 in colon cancer tissues and adjacent tissues, and to analyze the relationship between the expression of these two proteins and the clinicopathological features of colon cancer patients. The expression of SIRT1 and mutant p53 in colon cancer tissues was analyzed. Method 1: 1. The paraffin embedded tissues of 58 cases of carcinoma and 58 cases of paracancerous tissues were collected for HE staining. The expression of SIRT1 and mutant p53 in colon cancer and adjacent tissues were detected by immunohistochemical method. To analyze the relationship between the expression of SIRT1 and mutant p53 and the clinicopathological features of colon cancer. To analyze the correlation between SIRT1 and mutant p53 expression in colon cancer. Results: 1. SIRT1 was mainly located in the nucleus of colon cancer tissue, and a little in the cytoplasm. The positive expression rate of SIRT1 in cancer tissue was 43.1 / 58 and that in paracancerous tissue was 74.58%. The difference between the two groups was statistically significant (P 0.05 / 58). The positive expression rate of mutant p53 in colon cancer tissues was 51.7% and 30 / 58% respectively, and the positive expression rate in adjacent tissues was 0% / 58%. There was a significant difference between the two groups in the expression and age of SIRT1 in colon cancer. The degree of differentiation was related to P0.05, but not to sex, nationality, tumor size, depth of invasion, lymph node metastasis, TNM stage of distant metastasis. The expression of mutant p53 in colon cancer is related to age, sex, nationality, tumor size, differentiation, depth of invasion. There was no significant correlation between TNM stage of distant metastasis and mutant p53 expression in colon cancer tissues, and the correlation coefficient was 0.417% P ~ (0.05) P ~ (0.0010.05). 5. The expression of SIRT1 was positively correlated with the expression of mutant p53 in colon cancer tissues. Conclusion: 1. The overexpression of SIRT1 in colon cancer tissues is related to the age and differentiation degree of patients. 2. P53 expression in colon cancer tissue is higher than that in cancer tissue, and it is related to age. There is a positive correlation between SIRT1 and mutant p53 in colon cancer. SIRT1 and p53 may play a synergistic role in promoting the occurrence of colon cancer.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.35

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