IL-8和IL-10在卵巢癌中的表达及临床意义

发布时间：2018-05-16 13:40

本文选题：白介素8 + 白介素10　；参考：《青岛大学》2017年硕士论文

【摘要】：目的1.探讨卵巢癌组织中IL-8基因的表达及其与卵巢癌临床病理特征之间的关系。2.探讨卵巢癌组织中IL-10基因的表达及其与卵巢癌临床病理特征之间的关系。3.分析卵巢癌中IL-8和IL-10基因表达的相关性以及临床意义。方法1.分别选用免疫组织化学染色的方式从蛋白水平及逆转录-聚合酶链反应(RT-PCR)的方式从m RNA水平测验了实验组(60例卵巢癌组织)和对照组(30例良性卵巢肿瘤组织、8例正常卵巢组织)内IL-8与IL-10表达的不同。2.使用SPSS17.0软件对实验进行统计分析。-x±s被用来表示计量资料,t检验被用来对两组进行比较,Spearman相关系数被用来对相关性进行分析。Kaplan-Meier法被用来绘制生存曲线,Log-rank检验被用来比较生存率,多因素Cox比例风险回归模型被用来分析独立危险因素,P0.05表示差异有明显的统计学的意义。结果1.IL-8基因在卵巢肿瘤组织中的表达:免疫组化结果可知实验组IL-8蛋白呈现高表达,IL-8阳性率为65%(39/60)(P0.05),而对照组未检测到表达;RT-PCR结果显示实验组中IL-8 m RNA的阳性表达率(0.519士0.077)高于对照组中IL-8基因的表达率(0.319士0.077,0.141士0.023)(P0.05)。IL-8蛋白和m RNA水平的表达与卵巢癌临床分期、组织分化程度及是否淋巴结转移有关,IL-8蛋白和m RNA在III-IV期的阳性表达率分别为(78.05%,0.636±0.082)远远高于I-II期(36.84%,0.501±0.134)(P0.05),中、低分化组(77.27%,0.569±0.119)远远高于高分化组(31.25%,0.453±0.139)(P0.05),有淋巴结转移者(76.92%,0.581±0.115)远远高于无淋巴结转移者(42.86%,0.469±0.142)(P0.05),两者的表达水平与卵巢癌的病理类型无关(P0.05);IL-8阴性组(2.86年)生存时间明显长于阳性组(2.24年)(P0.01)。2.IL-10基因在卵巢肿瘤组织中的表达:免疫组化结果可知实验组IL-10蛋白呈现高表达,IL-10阳性率为63.33%(38/60)(P0.05);RT-PCR结果显示实验组中IL-10基因的阳性表达率(0.592士0.160)高于对照组中IL-10基因表达率(0.391士0.170,0.194土0.042)(P0.05);IL-10蛋白和m RNA水平的表达与卵巢癌临床分期、组织分化程度及是否淋巴结转移有关,IL-10蛋白和m RNA在III-IV期的阳性表达率分别为(78.05%,0.649±0.117)远远高于I-II期(31.58%,0.529±0.159)(P0.05),中、低分化组(72.73%,0.614±0.145)远远高于高分化组(37.50%,0.476±0.163)(P0.05),有淋巴结转移者(79.49%,0.620±0.142)远远高于无淋巴结转移者(33.33%,0.474±0.165)(P0.05),两者的表达水平与卵巢癌的病理类型无关(P0.05);IL-10阴性组(3.14年)生存时间明显长于阳性组(2.33年)(P0.01)。3.卵巢癌组织中IL-8和IL-10的表达具有正相关性(r=0.461,P0.05)。结论IL-8与IL-10在卵巢癌组织内基因表达率高,与卵巢癌临床病理特征有一定相关性,两者之间统计学分析呈正相关。其可能作为卵巢癌的分子生物学标志,成为卵巢癌治疗的新靶点。
[Abstract]:Objective 1. To investigate the expression of IL-8 gene and its relationship with clinicopathological features of ovarian cancer. To investigate the expression of IL-10 gene and its relationship with clinicopathological features of ovarian cancer. To analyze the correlation and clinical significance of IL-8 and IL-10 gene expression in ovarian cancer. Method 1. Immunohistochemical staining and reverse transcription-polymerase chain reaction RT-PCR were used to test 60 ovarian cancer tissues in the experimental group and 30 benign ovarian tumor tissues in the control group. The difference between IL-8 and IL-10 expression in normal ovarian tissue. The statistical analysis of the experiment using SPSS17.0 software .-x 卤s was used to indicate that the t test was used to compare the two groups. Kaplan-Meier method was used to analyze the correlation. Kaplan-Meier method was used to draw the survival curve. Log-rank test was used to compare the survival rate. Multivariate Cox proportional risk regression model was used to analyze independent risk factors. Results the expression of 1.IL-8 gene in ovarian neoplasms: the results of immunohistochemistry showed that the positive rate of IL-8 protein in the experimental group was 65 / 39 / 60 / 60 (P0.05A), while that of the control group was not detected by RT-PCR. The results of RT-PCR showed that the positive rate of IL-8 m RNA in the experimental group was higher than that in the control group. The expression rate of IL-8 gene was 0.319 + 0.077 + 0.141 + 0.023)(P0.05).IL-8 protein and m RNA and the clinical stage of ovarian cancer was significantly higher than that in control group (0.519 卤0.077). The positive expression rates of IL-8 protein and m RNA in the III-IV phase were significantly higher than those in the I-II stage (0.501 卤0.134), respectively, and the positive rates of IL-8 protein and m RNA were 0.636 卤0.082 and 0.501 卤0.134 respectively in the tissue differentiation and lymph node metastasis. The expression level in poorly differentiated group was much higher than that in well-differentiated group (0.453 卤0.139) P 0.05, and in lymph node metastasis group (76.92 卤0.115) was much higher than that in non-lymph node metastasis group (0.469 卤0.142P 0.0519). There was no correlation between the expression level and pathological type of ovarian cancer. The survival time of P0.05 + IL-8 negative group (2.86 years) was significantly longer than that of positive group (2.86 years). Expression of IL-10 gene in ovarian tumor tissues in 2.24 years: the results of immunohistochemistry showed that the positive rate of IL-10 protein in experimental group was 63.33% and the positive rate of IL-10 gene in experimental group was 63.33%. The positive rate of IL-10 gene in experimental group was 0.592 卤0.160), which was higher than that in control group. The expression of IL-10 protein and m RNA were correlated with the clinical stage of ovarian cancer. The positive expression rates of IL-10 protein and m RNA in the III-IV phase were significantly higher than those in the I-II stage (0. 529 卤0. 159) and 0. 649 卤0. 117 respectively. The expression of IL-10 in poorly differentiated group was significantly higher than that in well-differentiated group (0.476 卤0.163) and lymph node metastasis (79.490.20 卤0.142). It was significantly higher in poorly differentiated group than in non-lymph node metastasis group (0.474 卤0.165P0.05). The expression level was not related to the pathological type of ovarian cancer. The survival time of the negative group (3.14) was significantly longer than that of the positive group (2.33 years, P 0.01T. There was a positive correlation between the expression of IL-8 and IL-10 in ovarian cancer tissues. Conclusion the gene expression rate of IL-8 and IL-10 in ovarian cancer is high, and there is a certain correlation with clinicopathological characteristics of ovarian cancer, and there is a positive correlation between them. It may be a molecular marker of ovarian cancer and a new target for ovarian cancer therapy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.31

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