骨髓间充质干细胞与骨髓瘤细胞融合的实验研究

发布时间：2018-05-18 22:17

本文选题：骨髓间充质干细胞 + 骨髓瘤细胞　；参考：《苏州大学》2015年硕士论文

【摘要】：目的:体外构建骨髓间充质干细胞与骨髓瘤细胞的融合细胞,初步探讨融合细胞的生物学特性。方法:1.分别分离培养正常人及骨髓瘤患者的骨髓间充质干细胞(Mesenchymal Stem Cells,MSCs),并对其生物学特性行初步鉴定;2.细胞示踪绿色荧光探针(CMFDA)及红色荧光探针(CMTMR)分别标记MSCs及骨髓瘤细胞通过化学促融剂聚乙二醇(polyethylene glycol,PEG-1000)诱导融合,建立细胞融合模型;3融合细胞生物学特性研究:染色体核型分析,确定融合细胞是否发生细胞核的融合,鉴定融合细胞干性相关基因C-myc、Sox2、Oct-4等及促融相关性基因SIRPα及DC-STAMP的表达情况。结果:1.成功分离并培养人骨髓间充质干细胞;2.聚乙二醇(PEG-1000)能够介导MSCs与骨髓瘤细胞融合;3.超过80%的杂交细胞染色体数目在90条左右;融合细胞不仅表现出MSCs的特征,干性相关基因C-myc、Sox2、Oct-4表达阳性,而且融合相关性基因SIRPα及DC-STAMP也表达阳性。结论:MSCs与骨髓瘤细胞间可形成融合细胞,融合细胞具有进一步成融的潜力,是多发性骨髓瘤发生、发展的重要原因之一。
[Abstract]:Aim: to construct the fusion cells of bone marrow mesenchymal stem cells and myeloma cells in vitro and to explore the biological characteristics of the fusion cells. Method 1: 1. Bone marrow mesenchymal Stem cells were isolated from normal persons and patients with myeloma, and their biological characteristics were preliminarily identified. MSCs and myeloma cells were labeled with green fluorescent probe (CMFDA) and red fluorescent probe (CMT MR), respectively. The fusion was induced by polyethylene glycol polyethylene glycolen (PEG-1000), and the cell fusion model was established. The biological characteristics of fusion cells were studied: chromosome karyotype analysis. To determine whether the fusion cells have nuclear fusion, and to identify the expression of C-mycton-Sox2Oct-4 and SIRP 伪 and DC-STAMP in fusion cells. The result is 1: 1. Human bone marrow mesenchymal stem cells were isolated and cultured successfully. PEG-1000) can mediate the fusion of MSCs with myeloma cells. The chromosome number of more than 80% of the hybrid cells was about 90, and the fusion cells not only showed the characteristics of MSCs, but also expressed the fusion genes SIRP 伪 and DC-STAMP. Conclusion the fusion cells can be formed between MSCs and myeloma cells, and the fusion cells have the potential of further fusion, which is one of the important reasons for the occurrence and development of multiple myeloma.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R733.3

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