T肽增强顺铂肿瘤杀伤作用的研究及机制探讨

发布时间：2018-05-18 21:44

本文选题：肺肿瘤 + T肽　；参考：《中国肺癌杂志》2017年02期

【摘要】：背景与目的 T肽是Tuftsin的衍生物,其基本功能是刺激巨噬细胞,提高巨噬细胞吞噬及分泌能力,通过增强效应细胞的细胞杀伤作用而被广泛用于多种恶性肿瘤术后抗肿瘤治疗。本研究旨在探讨T肽与化疗药物顺铂的联合能否提高铂类药物的抗肿瘤疗效及机制。方法应用酶联免疫吸附剂测定(enzyme-linked immunosorbent assay,ELISA)T肽和/或顺铂处理人巨噬细胞株U937后肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和干扰素-γ(interferon-γ,IFN-γ)分泌水平的变化;应用裸鼠荷瘤动物模型,分析T肽和/或顺铂处理小鼠肿瘤变化情况以及小鼠用药期间外周血中巨噬细胞变化情况。结果 (1)应用ELISA检测发现在T肽组和T肽联合顺铂组中,U937细胞培养液中TNF-α水平明显高于对照组和顺铂组;T肽联合顺铂组中IFN-γ水平明显高于对照组、顺铂组和T肽组;(2)在小鼠荷瘤模型中,T肽联合顺铂组的肿瘤体积明显小于对照组、顺铂组和T肽组,并且小鼠没有明显的体重下降;(3)在小鼠外周血的检测分析中发现,T肽联合顺铂组中活化巨噬细胞数呈现增长趋势。免疫组化分析各组中肿瘤组织Ki67显示T肽联合顺铂组抑制肿瘤细胞增殖。结论 T肽能够促进巨噬细胞增殖和促进其分泌肿瘤细胞杀伤因子(TNF-α、IFN-γ);在体内动物模型中,T肽能够提高化疗药物如顺铂的疗效,使联合组具有更强的肿瘤抑制作用,同时能够减轻化疗药物的毒副作用。
[Abstract]:Background & objective T peptide is a derivative of Tuftsin. Its basic function is to stimulate macrophage, enhance phagocytosis and secretion of macrophage, and is widely used in anti-tumor therapy after operation of various malignant tumors by enhancing the cytotoxicity of effector cells. The aim of this study was to investigate whether the combination of T peptide and cisplatin could improve the anti-tumor effect of platinum. Methods Enzyme-linked immunosorbent assay (Elisa) was used to determine the secretion of tumor necrosis factor 伪 tumor necrosis factor- 伪 (TNF- 伪) and interferon- 纬 (IFN- 纬) in human macrophage cell line U937 treated with Enzyme-linked immunosorbent Assay T peptide and / or cisplatin. The changes of tumor and macrophages in peripheral blood of mice treated with T peptide and / or cisplatin were analyzed. Results (1) the levels of TNF- 伪 in the culture medium of U937 cells in T peptide group and T peptide combined with cisplatin group were significantly higher than those in control group and cisplatin combined with cisplatin group, and the level of IFN- 纬 in T peptide combined with cisplatin group was significantly higher than that in control group. Cisplatin group and T peptide group were significantly smaller than the control group in tumor bearing model, and the tumor volume in Cisplatin group and T peptide group was significantly lower than that in control group, and the tumor volume in Cisplatin group and T peptide group was significantly lower than that in control group. In addition, no significant weight loss was found in mice) the number of activated macrophages in the combination of T peptide and cisplatin group showed an increasing trend in the detection and analysis of peripheral blood of mice. Immunohistochemical analysis showed that T peptide combined with cisplatin inhibited the proliferation of tumor cells in Ki67. Conclusion T peptide can promote the proliferation of macrophages and the secretion of tumor killer factor TNF- 伪 -IFN- 纬, in vivo animal model, T peptide can improve the therapeutic effect of chemotherapy drugs such as cisplatin, and make the combined group have stronger tumor inhibitory effect. At the same time, it can reduce the side effects of chemotherapeutic drugs.
【作者单位】：天津医科大学总医院肺部肿瘤外科;天津市肺癌研究所天津市肺癌转移与肿瘤微环境实验室;
【基金】：国家自然科学基金项目(No.81172233,No.81372306) 天津自然科学基金重点项目(No.16JCZDJC34200);天津自然科学基金项目(No.13JCYBJC22600) 教育部高等学校博士学科点专项科研基金项目(No.20131202110004) 天津市创新平台专项市级重点实验室建设项目(No.16PTSYJC00160)资助~~
【分类号】：R734.2

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