PEDF对人脐静脉内皮细胞和肺癌SK-MES-1细胞增殖的影响及作用机制

发布时间：2018-05-19 16:47

本文选题：色素上皮衍生因子 + 脐静脉内皮细胞　；参考：《安徽医科大学学报》2017年05期

【摘要】：目的探讨色素上皮衍生因子(PEDF)对SK-MES-1细胞和人脐静脉内皮细胞(HUVECs)增殖的影响及可能的机制。方法 CCK-8法检测不同浓度PEDF在不同作用时间条件下对HUVECs和SK-MES-1细胞增殖的影响;流式细胞仪检测不同浓度PEDF对此两种细胞凋亡的影响;qRTPCR检测PEDF对此两种细胞中血管内皮生长因子(VEGF)基因表达水平的影响。结果 CCK-8结果显示,PEDF对HUVECs和SK-MES-1细胞具有增殖抑制作用,呈一定浓度和时间依赖性(P0.05);流式细胞术结果表明,实验组细胞的凋亡率高于对照组(P0.05),高浓度用药组凋亡率高于低浓度用药组(P0.05);qRT-PCR结果表明,与对照组相比,PEDF能抑制HUVECs和SK-MES-1细胞中VEGF mRNA水平的表达(P0.05)。结论 PEDF的抗肿瘤作用主要包括抑制肿瘤血管生成和直接作用于肿瘤细胞两方面,PEDF对HUVECs和SK-MES-1细胞增殖的影响可能与降低VEGF表达水平有关。
[Abstract]:Objective to investigate the effect and possible mechanism of pigment epithelium-derived factor (PEDF) on proliferation of SK-MES-1 cells and human umbilical vein endothelial cells (HUVECs). Methods CCK-8 assay was used to detect the effects of different concentrations of PEDF on the proliferation of HUVECs and SK-MES-1 cells. Effects of different concentrations of PEDF on apoptosis of these two cells the effect of PEDF on the expression of VEGF gene was detected by flow cytometry. Results the results of CCK-8 showed that the proliferation of HUVECs and SK-MES-1 cells was inhibited in a dose-and time-dependent manner, and the flow cytometry showed that the proliferation of HUVECs and SK-MES-1 cells was inhibited in a dose-and time-dependent manner. The apoptotic rate in the experimental group was higher than that in the control group (P 0.05), and the apoptosis rate in the high concentration group was higher than that in the low concentration group. The results of RT-PCR showed that PEDF could inhibit the expression of VEGF mRNA in HUVECs and SK-MES-1 cells. Conclusion the antitumor effects of PEDF mainly include inhibiting tumor angiogenesis and directly acting on tumor cells. The effect of PEDF on the proliferation of HUVECs and SK-MES-1 cells may be related to the decrease of VEGF expression level.
【作者单位】：安徽医科大学第四附属医院呼吸内科;
【基金】：安徽省自然科学基金面上项目(编号:1308085MH141) 安徽医科大学科研基金资助项目(编号:2010 xkj115)
【分类号】：R734.2

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