Raptor通过上皮-间质转化促进乳腺癌的侵袭与转移

发布时间：2018-05-22 17:06

本文选题：Raptor + 上皮-间质转化　；参考：《中国药理学通报》2017年08期

【摘要】：目的探讨Raptor在乳腺癌侵袭与转移过程中的作用。方法采用Western blot检测乳腺癌细胞MCF-7和MDA-MB-231(MDA231)中Raptor蛋白的表达情况。应用小干扰RNA技术下调乳腺癌细胞MDA231中Raptor的表达量,同时将过表达质粒转入乳腺癌细胞MCF-7中,应用Western blot法检测转染后各组细胞中上皮性钙黏着蛋白(E-cadherin)和波形蛋白(Vimentin)的表达;应用Transwell体外侵袭实验检测转染后各组细胞的侵袭能力;应用细胞核质分离实验检测转染后各组细胞中Snail蛋白的转核情况。结果MCF-7细胞中Raptor蛋白的表达量明显低于MDA231细胞中的表达量;转染后,与对照组Scr/MDA231相比,siRaptor/MDA231组中Raptor蛋白的表达量明显降低,Vimentin蛋白表达量降低,而E-cadherin蛋白表达量升高;与对照组MCF-7/Con相比,MCF-7/Raptor组中Raptor蛋白的表达量明显升高,并伴有Vimentin蛋白表达量升高,而Ecadherin蛋白表达量降低。siRaptor/MDA231细胞组中穿过Matrigel小室的细胞数量明显减少(P0.05);MCF-7/Raptor细胞组中穿过Matrigel小室的细胞数量明显升高(P0.05)。细胞核质分离实验结果显示,siRaptor/MDA231组中Snail蛋白表达量较Scr/MDA231组中明显降低;MCF-7/Raptor组中Snail蛋白表达量较MCF-7/Con组中明显升高。结论 Raptor能够促进乳腺癌EMT的发生,对乳腺癌的侵袭与转移有重要作用。
[Abstract]:Objective to investigate the role of Raptor in the invasion and metastasis of breast cancer. Methods Western blot was used to detect the expression of Raptor protein in breast cancer cells MCF-7 and MDA-MB-231. The expression of Raptor in breast cancer cell line MDA231 was down-regulated by small interfering RNA technique, and the overexpression plasmid was transferred into the breast cancer cell MCF-7. The expression of E-cadherin and vimentin in the transfected cells was detected by Western blot assay. Transwell invasion assay in vitro was used to detect the invasiveness of the transfected cells, and the nuclear cytoplasmic separation assay was used to detect the transnucleation of Snail protein in the transfected cells. Results the expression of Raptor protein in MCF-7 cells was significantly lower than that in MDA231 cells, and after transfection, the expression of Raptor protein in Raptor / MDA231 group was significantly lower than that in control Scr/MDA231 group, but the expression of E-cadherin protein was increased. Compared with the control group, the expression of Raptor protein in MCF-7 / Raptor group was significantly increased, and the expression of Vimentin protein was increased in MCF-7 / Raptor group. However, the number of cells passing through the Matrigel chamber in the siraptor / MDA231 cell group was significantly decreased, while the number of cells passing through the Matrigel chamber in the MCF-7 / Raptor cell group was significantly increased (P 0.05). The results of nuclear cytoplasmic separation test showed that the expression of Snail protein in MDA231 group was significantly lower than that in Scr/MDA231 group. The expression of Snail protein in MCF-7 / Raptor group was significantly higher than that in MCF-7/Con group. Conclusion Raptor can promote the occurrence of EMT in breast cancer and play an important role in invasion and metastasis of breast cancer.
【作者单位】：潍坊医学院病理学教研室;潍坊医学院医学研究实验中心;潍坊医学院护理学院;
【基金】：国家自然科学基金青年基金项目(No 81402389,81641111) 山东省自然科学基金资助项目(No ZR2014HL077,ZR 2015HL065) 山东省高等学校科技计划(No J12LK03,J13LK03)
【分类号】：R737.9

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