同步荧光光谱法研究肿瘤芳香族氨基酸残基含量的变化

发布时间：2018-05-23 14:19

  本文选题：同步荧光光谱 + 色氨酸残基　； 参考：《光谱学与光谱分析》2017年09期

【摘要】：用同步荧光光谱法评估原发性肝细胞癌患者血浆、肝癌荷瘤小鼠以及培养细胞(HepG2和HL-7702)中酪氨酸(Tyr)和色氨酸(Trp)残基水平变化。固定发射波长λem和激发波长λex之间的波长差Δλ分别为20和60nm,激发和发射单色器同时进行扫描,确定350nm为Trp的同步特征发射峰位置,318nm为Tyr的同步特征发射峰位置。结果表明,肝癌患者及荷瘤小鼠血浆蛋白质所含Tyr和Trp残基的荧光强度明显增加。相反,肝癌细胞或荷瘤小鼠肿瘤组织中Tyr和Trp残基荧光强度却随生长时间增长而减少。进一步实验表明,具有抗癌活性的苦参碱处理癌细胞后,细胞Tyr和Trp残基的荧光强度升高。这些结果表明,Tyr和Trp残基的变化可能参与了肿瘤的发生发展。
[Abstract]:The changes of tyrosine (TRP) and tryptophan (Trp) residues in plasma of hepatocellular carcinoma (HCC) mice and cultured cells (HepG2 and HL-7702) were evaluated by synchronous fluorescence spectrometry. The wavelength difference 螖 位 between constant emission wavelength 位 em and excitation wavelength 位 ex is 20 and 60 nm, respectively. The excitation and emission Monochromator scan at the same time and determine that 350nm is the position of synchronous characteristic emission peak of Trp and position of synchronous characteristic emission peak of Tyr. The results showed that the fluorescence intensity of Tyr and Trp residues in plasma proteins of HCC patients and tumor-bearing mice were significantly increased. In contrast, the fluorescence intensity of Tyr and Trp residues in tumor tissues of hepatoma cells or tumor-bearing mice decreased with the increase of growth time. Further experiments showed that the fluorescence intensity of Tyr and Trp residues increased after matrine treatment with anticancer activity. These results suggest that the changes of Tyr and Trp residues may be involved in tumorigenesis and progression.
【作者单位】： 汕头大学医学院生物分析实验室;汕头大学医学院第二附属医院急诊科;
【基金】：广东省科学技术厅-广东省中医药科学院联合科研专项(2012A032500009)资助
【分类号】：O657.3;R730.43
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本文编号：1925115