CUGBP1过表达与非小细胞肺癌发生发展相关性的研究

发布时间：2018-05-24 18:49

本文选题：非小细胞肺癌 + CUGBP1　；参考：《青岛大学》2015年硕士论文

【摘要】：【背景】CUGBP1是一种多功能RNA结合蛋白,可调节胞核及胞质内m RNA的剪接、翻译、降解多种作用,并可维持其稳定性。既往有研究表明CUGBP1在非小细胞肺癌中过表达,但是在肿瘤的发生和发展的具体病理作用仍不清楚。【方法】本实验应用免疫组化法检测CUGBP1在未接受过任何化放疗治疗的80例非小细胞肺癌的肿瘤组织及癌旁组织中的差异性表达,然后慢病毒转染、Western Blot法、CCK-8法、流式细胞术和DAPI染色,探讨CUGBP1在非小细胞肺癌细胞株A549的增殖、凋亡及上皮间质转化(EMT)过程中的生物学作用。【结果】采用免疫组化法显示,在非小细胞肺癌的肿瘤组织及癌旁组织中存在CUGBP1的表达差异。在非小细胞肺癌A549细胞株中,CUGBP1经sh-RNA沉默后可促进细胞的凋亡,降低细胞的增值能力。此外,Western blot分析表明,沉默CUGBP1后可增加细胞周期蛋白D1,BAD,Jun D及E-cadherin的表达,同时可抑制细胞周期蛋白B1。此外,CUGBP1沉默后可下调β-catenin和vimentin水平,上调E-cadherin水平,这提示CUGBP1可能与上皮间质转化的过程有关。【结论】该研究表明在非小细胞肺癌的生理与病理过程中CUGBP1发挥着相当重要的作用,并可作为非小细胞肺癌治疗的一个潜在靶点。
[Abstract]:[background] CUGBP1 is a multifunctional RNA binding protein that regulates splicing, translation, degradation and stability of m RNA in nucleus and cytoplasm. Previous studies have shown that CUGBP1 is overexpressed in non-small cell lung cancer. However, the specific pathological role of tumorigenesis and development is still unclear. [methods] Immunohistochemical method was used to detect CUGBP1 in 80 cases of non-small cell lung cancer (NSCLC) without any chemotherapeutic therapy. The differential expression in weaving, Then the lentivirus was transfected with CCK-8, flow cytometry and DAPI staining to investigate the biological role of CUGBP1 in the proliferation, apoptosis and epithelial mesenchymal transformation of non-small cell lung cancer cell line A549. [results] Immunohistochemical method was used to demonstrate the effect of CUGBP1 on the proliferation, apoptosis and epithelial mesenchymal transformation of human lung cancer cell line A549. There is a difference in the expression of CUGBP1 in tumor tissues and adjacent tissues of non-small cell lung cancer (NSCLC). In A549 cell line of non-small cell lung cancer (NSCLC), CUGBP1 silenced by sh-RNA could promote cell apoptosis and decrease cell proliferation. In addition, Western blot analysis showed that silencing CUGBP1 could increase the expression of cyclin D 1 BADN Jun D and E-cadherin, and inhibit the expression of cyclin B 1. In addition, CUGBP1 can down-regulate 尾 -catenin and vimentin levels and up-regulate E-cadherin levels after silencing, suggesting that CUGBP1 may be involved in the process of epithelial interstitial transformation. [conclusion] this study suggests that CUGBP1 plays an important role in the physiological and pathological processes of non-small cell lung cancer. It can be used as a potential target for the treatment of non-small cell lung cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R734.2

【相似文献】