小鼠自发乳腺癌模型中肿瘤相关巨噬细胞的表型检测与分析

发布时间：2018-05-25 04:02

本文选题：乳腺癌 + 肿瘤相关巨噬细胞(TAM)　；参考：《细胞与分子免疫学杂志》2017年06期

【摘要】：目的通过检测巨噬细胞极化标志性分子表达水平变化分析自发乳腺癌进程中肿瘤相关巨噬细胞(TAM)的表型转化。方法通过基因型鉴定获得自发乳腺癌模型小鼠,并通过检测其肿瘤总体积变化,将小鼠分为肿瘤早期组及晚期组;通过机械法获得肿瘤组织单细胞悬液并通过流式细胞分选获得不同病程的TAM;利用实时定量PCR检测M1型巨噬细胞白细胞介素1β(IL-1β)、IL-27、IL-6、CD80、CD86和M2型巨噬细胞标志性分子精氨酸酶1(Arg1)、IL-10、白细胞介素4受体α(IL-4Rα)、巨噬细胞甘露糖受体1(Mrc1)、几丁质酶样分子3(Chil3/Ym1)水平;通过对比早期与晚期肿瘤组织巨噬细胞极化标志性分子的表达倾向性,分析TAM在肿瘤不同时期的表型。结果早期肿瘤的TAM高表达部分M1型巨噬细胞标志性分子IL-1β、CD80、CD86,晚期肿瘤的TAM高表达IL-6及部分M2型巨噬细胞极化标志性分子Arg1、IL-10、IL-4Rα。结论肿瘤进程中,TAM经历表型转换过程,靶向TAM的表型转换有可能为乳腺癌提供新的治疗方案。
[Abstract]:Objective to investigate the phenotypic transformation of tumor associated macrophages (TAM) in the process of spontaneous breast cancer by detecting the expression level of macrophage polarization markers. Methods spontaneous breast cancer model mice were obtained by genotypic identification. The mice were divided into early group and late group by measuring the total tumor volume. Single cell suspensions of tumor tissue were obtained by mechanical method and TAMs of different course were obtained by flow cytometry, and interleukin-1 尾 (IL-1 尾) of M1 macrophages were detected by real-time quantitative PCR, CD86 and arginase, the iconic molecules of macrophage type M 2 and IL-27, IL-6, CD80 and CD86, respectively, were detected by real-time quantitative PCR. 1the levels of IL-10, IL-4 receptor 伪 -IL-4R 伪, macrophage mannose receptor 1mc1, chitinase like molecule 3Chil3 / YM1); The phenotypes of TAM at different stages of tumor were analyzed by comparing the expression tendency of macrophage polarization markers in early and late stage of tumor. Results the TAM expression of partial M1 macrophages was high in the early stage, and the expression of IL-6 in TAM and IL-4R 伪 in some M 2 macrophages was higher than that in the advanced tumors. Conclusion the phenotypic transition of TAM may provide a new therapy for breast cancer.
【作者单位】：第三军医大学;解放军总医院骨科;
【基金】：国家自然科学基金(81402216)
【分类号】：R-332;R737.9

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