肿瘤坏死因子相关凋亡诱导配体及其与5-Fu联合用药对T-24细胞生长的抑制作用

发布时间：2018-05-25 20:38

本文选题：TRAIL + T-　；参考：《中国老年学杂志》2017年23期

【摘要】：目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)单独应用及其与传统化疗药物5-氟尿嘧啶(5-Fu)联合应用对体外培养人膀胱肿瘤T-24细胞生物学行为影响及可能机制。方法倒置显微镜下观察不同浓度TRAIL作用不同时间后T-24细胞形态变化;MTT法检测不同浓度TRAIL单独或10 ng/ml TRAIL联合不同浓度5-Fu不同时间后T-24细胞生存率;流式细胞仪检测T-24细胞凋亡率。结果 TRAIL单独应用时对T-24细胞生长抑制作用有限,显微镜下部分细胞出现形态学改变,作用72 h后1、300、1 000 ng/ml组细胞生存率分别(92.52±3.60)%,(84.63±5.93)%和(79.45±2.04)%;单用200、400、600、800及1 000μmol/ml 5-Fu时细胞生长抑制率分别为(10.76±1.78)%、(14.17±2.75)%、(28.01±1.66)%、(20.17±1.68)%和(32.53±3.65)%,联合10 ng/ml TRAIL处理后0、200、400、600、800及1 000μmol/ml 5-Fu组细胞抑制率分别为分别为(3.85±0.88)%、(8.41±0.74)%、(16.39±2.72)%、(26.27±1.72)%、(28.99±3.36)%和(30.18±1.34)%;10、100、1 000 ng/ml TRAIL处理48 h后的细胞平均凋亡率为(3.75±0.31)%、(7.82±1.07)%、(7.78±3.15)%。结论 TRAIL单用或联合其他化疗药物对T24细胞生长抑制作用有限,同5-Fu联合应用时不能明显增加T-24细胞对5-Fu的敏感性。
[Abstract]:Objective to study the application of tumor necrosis factor related apoptosis inducing ligand (TRAIL) alone and its combination with the traditional chemotherapy drug 5- fluorouracil (5-Fu) on the biological behavior of human bladder tumor T-24 cells in vitro and its possible mechanism. The morphological changes of T-24 cells after different concentrations of TRAIL at different concentrations were observed under the inverted microscope. MTT assay was used to detect the survival rate of T-24 cells after different concentrations of different concentrations of TRAIL alone or 10 ng/ml TRAIL combined with different concentrations of 5-Fu; flow cytometry was used to detect the apoptosis rate of T-24 cells. Results the inhibitory effect of TRAIL on T-24 cell growth was limited when TRAIL was used alone, morphologic changes in some cells under microscope, and 1300,1 000 ng/ml groups after the action of 72 h The cell survival rate was (92.52 + 3.60)%, (84.63 + 5.93)% and (79.45 + 2.04)%, and the growth inhibition rate of cell growth was (10.76 + 1.78)%, (14.17 + 2.75)%, (14.17 + 2.75)%, (28.01 + 84.63)%, (14.17 + 2.75)%, (2.04%)% and (79.45%)% respectively. Cell inhibition of combined ng/ml TRAIL and mol/ml 5-Fu group cell inhibition The rates were (3.85 + 0.88)%, (8.41 + 0.74)%, (16.39 + 2.72)%, (26.27 + 1.72)%, (28.99 + 3.36)% and (30.18 + 1.34)%, and the average apoptosis rate of cells after 10100,1 000 ng/ml TRAIL treatment was +%%, and the inhibitory effect of TRAIL alone or combined with other chemotherapeutic drugs was limited, When combined with 5-Fu, the sensitivity of T-24 cells to 5-Fu could not be significantly increased.
【作者单位】：三峡大学第一临床医学院泌尿外科三峡大学泌尿外科研究所;三峡大学仁和医院;
【分类号】：R737.14

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