抑制自噬增强门冬酰胺酶对小细胞肺癌细胞H1688和H446的抗癌作用

发布时间：2018-05-27 17:22

  本文选题：小细胞肺癌 + 门冬酰胺酶　； 参考：《第三军医大学学报》2017年06期

【摘要】：目的研究门冬酰胺酶(asparaginase,AN)对小细胞肺癌H1688和H446细胞的抗癌作用,探讨自噬在AN抗癌过程中的功能。方法采用MTT法及台盼蓝染色法检测AN单独或联用自噬抑制剂氯喹(chloroquine,CQ)对H1688和H446细胞的生长抑制作用;免疫荧光法观察自噬标记物LC3表达,示踪自噬体形成;Western blot检测自噬相关蛋白LC3及Akt/m TOR信号通路的表达。结果 AN呈浓度依赖性抑制H1688和H446细胞增殖并促进其死亡(P0.05);AN处理组可以显著增加H1688和H446细胞自噬小体数量并诱导LC3-Ⅱ表达;自噬抑制剂CQ提高AN对H1688和H446细胞杀伤作用(P0.05);AN作用H1688细胞后p-Akt、p-m TOR、p-70S6K蛋白表达降低。结论 AN对小细胞肺癌H1688和H446细胞具有抑制作用并诱导细胞保护性自噬,阻断自噬可以增强AN对小细胞肺癌H1688和H446细胞的抗癌疗效。
[Abstract]:Objective to study the anticancer effects of asparagase ANs on H1688 and H446 cells of small cell lung cancer and to investigate the function of autophagy in the anticancer process of an. Methods MTT assay and Trypan blue staining were used to detect the growth inhibition of H1688 and H446 cells by an alone or in combination with chloroquine CQ, and the expression of autophagy marker LC3 was observed by immunofluorescence assay. The expression of autophagy associated protein (LC3) and Akt/m TOR signal pathway was detected by Western blot. Results an inhibited the proliferation of H1688 and H446 cells in a concentration-dependent manner and promoted the proliferation of H1688 and H446 cells and induced the expression of LC3- 鈪，

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