宫颈鳞状细胞癌组织LncRNA EXOC7表达与预后相关性研究

发布时间：2018-05-27 19:31

本文选题：宫颈鳞状细胞癌 + LncRNA　；参考：《中华肿瘤防治杂志》2017年16期

【摘要】：目的宫颈癌是女性生殖系统常见的恶性肿瘤之一。长链非编码RNA(lncRNA)的异常表达与肿瘤的发生、发展密切相关。本研究拟探讨长链非编码RNA外泌体复合物7(Long non-coding RNA Exocyst complex component 7,LncRNA EXOC7)在宫颈鳞状细胞癌(cervical squamous cell cancer,SCC)组织中的表达及临床意义。方法收集2012-01-01-2016-12-30四川省人民医院肿瘤科及妇产科行手术及穿刺活检的98例SCC患者组织标本,其中癌旁组织(距癌组织2cm)58例;同期收集该院40例正常宫颈组织。采用QRT-PCR法检测SCC组织及癌旁组织标本中LncRNA EXOC7的表达,采用单因素及多因素Cox回归分析LncRNA EXOC7的表达与患者临床病理特征及预后的关系,生存曲线分析LncRNA EXOC7与患者总生存时间(overall survival,OS)及无进展生存时间(progression free survival,PFS)的关系。结果 SCC组织中lncRNA EXOC7的平均表达量为(6.805±1.475),明显高于癌旁组织(1.510±0.860)及正常宫颈组织(1.476±0.33)的平均表达量,差异有统计学意义,F=74.43,P0.001。LncRNA EXOC7高表达与患者的FIGO分期、淋巴结转移和肿瘤浸润深度相关,差异均有统计学意义,均P0.05;高表达组LncRNA EXOC7患者PFS为(12.00±1.55)个月,较低表达组(27.35±2.25)个月明显缩短,差异有统计学意义,χ2=51.650,P0.001;高表达组LncRNA EXOC7患者OS为(25.08±3.32)个月,较低表达组(44.80±2.92)个月明显缩短,差异有统计学意义,χ2=38.23,P0.001;Cox多因素分析显示,LncRNA EXOC7的表达是SCC患者的独立预后因素,HR=3.750,95%CI为1.530~7.97,P0.001。结论高表达LncRNA EXOC7SCC患者的预后差,LncRNA EXOC7可能作为潜在的SCC治疗靶点及新预后评估分子标志物。
[Abstract]:Objective Cervical cancer is one of the most common malignant tumors in the female reproductive system. The abnormal expression of long chain non-coding RNAs lncRNAs is closely related to the occurrence and development of tumors. The purpose of this study was to investigate the expression and clinical significance of long chain noncoding RNA exosome complex 7(Long non-coding RNA Exocyst complex component 7 (LncRNA exoc7) in cervical squamous cell carcinoma (Cervical squamous cell cancerous SCC). Methods 98 specimens of SCC tissues from oncology department, department of gynecology and obstetrics and gynecology and obstetrics of Sichuan Provincial people's Hospital were collected from 2012-01-01-2016-12-30, including paracancerous tissue (2cm)58) and normal cervical tissue (40 cases). The expression of LncRNA EXOC7 in SCC and paracancerous tissues was detected by QRT-PCR method. The relationship between the expression of LncRNA EXOC7 and clinicopathological features and prognosis was analyzed by univariate and multivariate Cox regression analysis. Survival curve was used to analyze the relationship between LncRNA EXOC7 and total survival time (LncRNA EXOC7) and progression free survival (PFSs). Results the average expression of lncRNA EXOC7 in SCC was 6.805 卤1.475m, which was significantly higher than that in adjacent tissues (1.510 卤0.860) and normal cervix (1.476 卤0.33). The difference was statistically significant (P 0.001. 001.LncRNA EXOC7 expression was correlated with the FIGO stage, lymph node metastasis and depth of tumor invasion. The difference was statistically significant (P 0.05), the PFS of LncRNA EXOC7 patients with high expression was 12.00 卤1.55 months, which was significantly shorter than that of low expression group (27.35 卤2.25) months, the difference was statistically significant (蠂 ~ (2) 2) 51.650 (P 0.001), the OS of high expression group of LncRNA EXOC7 patients was 25.08 卤3.32 months, which was significantly shorter than that of low expression group (44.80 卤2.92) months. The multivariate analysis showed that the expression of LncRNA EXOC7 was an independent prognostic factor in patients with SCC. Conclusion the poor prognosis of LncRNA EXOC7SCC patients with high expression of LncRNA EXOC7 may be a potential therapeutic target for SCC and a new molecular marker for prognosis evaluation.
【作者单位】：四川省医学科学院·四川省人民医院肿瘤中心;
【基金】：国家自然基金青年项目(81301910)
【分类号】：R737.33

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