全反式维甲酸联合LY294002对食管癌EC1细胞增殖、迁移及PI3K、MMP-2表达的影响

发布时间：2018-05-27 19:53

本文选题：全反式维甲酸 + LY　；参考：《郑州大学学报(医学版)》2016年01期

【摘要】：目的:探讨全反式维甲酸(ATRA)、磷脂酰肌醇激酶-3(PI3K)抑制剂LY294002单独或联合作用对食管癌EC1细胞增殖、迁移及PI3K和MMP-2表达的影响。方法:将常规培养的EC1细胞随机分为ATRA组、LY294002组、ATRA+LY294002组和对照组4组,采用CCK-8检测各组细胞的增殖情况,通过划痕实验测定各组细胞的迁移能力,采用RT-PCR和Western blot方法检测各组细胞中PI3K及MMP-3 mRNA和蛋白的表达情况。结果:作用24、48和72 h后,ATRA+LY294002组的细胞增殖抑制率均高于其他2组(P0.05);作用24 h后,ATRA+LY294002组细胞迁移能力均低于其他3组(P0.05);作用48 h后,ATRA+LY294002组细胞PI3K及MMP-2 mRNA和蛋白的表达量均低于其他3组(P0.05)。结论:ATRA联合LY294002可协同抑制EC1细胞的增殖、迁移和PI3K、MMP-2的表达,进而可能协同抑制肿瘤血管生成。
[Abstract]:Aim: to investigate the effects of LY294002, an inhibitor of all trans retinoic acid (ATRA) and phosphatidylinositol kinase (PI3K), on the proliferation, migration and expression of PI3K and MMP-2 in esophageal carcinoma EC1 cells. Methods: the cultured EC1 cells were randomly divided into ATRA group (LY294002) and control group (4 groups). The proliferation of cells in each group was detected by CCK-8, and the migration ability of each group was measured by scratch test. The expression of PI3K, MMP-3 mRNA and protein was detected by RT-PCR and Western blot. Results: the cell proliferation inhibition rate of ATRA LY294002 group was higher than that of the other two groups after 24 h and 72 h, the migration ability of ATRA LY294002 group was lower than that of the other three groups after 24 h exposure, and the cell PI3K, MMP-2 mRNA and protein surface of ATRA LY294002 group after 48 h treatment were lower than that of the other three groups. The amount reached was lower than that of the other three groups (P 0.05). Conclusion LY294002 combined with TRA can synergistically inhibit the proliferation, migration and expression of PI3KfMMP-2 in EC1 cells, which may result in synergistic inhibition of tumor angiogenesis.
【作者单位】：郑州大学第一附属医院肿瘤科;焦作市第二人民医院肿瘤科;
【基金】：河南省科技攻关计划项目112102310154
【分类号】：R735.1

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