鞘糖脂抑制剂NB-DNJ对多发性骨髓瘤U266细胞RANKL及IL-6表达的影响

发布时间：2018-05-28 05:02

本文选题：多发性骨髓瘤骨病 + NB-DNJ　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:本实验研究旨在探索讨论体外鞘糖脂抑制剂NB-DNJ对多发性骨髓瘤U266细胞RANKL、IL-6表达的影响,并且比较不同浓度鞘糖脂抑制剂NB-DNJ与硼替佐米在人体外对MBD的影响。方法:第一部分:培养人骨髓瘤U266细胞,加入不同浓度鞘糖脂抑制剂NB-DNJ以及硼替佐米阳性干预药物。第二部分:用Real-time PCR检测不同浓度鞘糖脂抑制剂NB-DNJ与干预药物硼替佐米作用于U266细胞RANKL、IL-6的基因表达水平。第三部分:用Western blot检测不同浓度鞘糖脂抑制剂NB-DNJ与干预药物硼替佐米作用于U266细胞RANKL、IL-6的蛋白表达水平。结果:经不同浓度(5umol/L,50umol/L,500umol/L)NB-DNJ作用于U266细胞,其对RANKL和IL-6的m RNA及蛋白表达的抑制作用呈剂量依赖性(P0.05)。研究发现500umol/LNB-DNJ组与硼替佐米干预组对RANKL的mRNA及蛋白表达,差异无统计学意义(P0.05),但对IL-6的mRNA及蛋白表达抑制作用较硼替佐米强(P0.05)。结论:鞘糖脂抑制剂NB-DNJ影响MM破骨活化因子的表达;体外证实鞘糖脂抑制剂NB-DNJ对MBD的影响效果优于硼替佐米。
[Abstract]:Aim: to explore the effect of NB-DNJ on the expression of RANKLLX IL-6 in multiple myeloma U266 cells in vitro, and to compare the effects of different concentrations of sphingolipids inhibitor NB-DNJ and bortezomil on MBD in vitro. Methods: human myeloma U266 cells were cultured with different concentrations of sphingolipids inhibitor (NB-DNJ) and bortezomil positive drugs. Part two: Real-time PCR was used to detect the gene expression level of RANKLX IL-6 in U266 cells treated with different concentrations of sphingolipids inhibitor NB-DNJ and bortezomil. Part three: Western blot was used to detect the protein expression level of RANKL- 6-6 in U266 cells treated with different concentrations of sphingolipids inhibitor NB-DNJ and bortezomil. Results: NB-DNJ was treated with NB-DNJ in U266 cells at different concentrations of 5umolr / L ~ 50umol/ L ~ (50) 渭 mol / L ~ (-1). The inhibitory effect of NB-DNJ on the expression of m RNA and protein of RANKL and IL-6 was in a dose-dependent manner (P0.05). It was found that there was no significant difference in the expression of mRNA and protein in RANKL between 500umol/LNB-DNJ group and bortezomil intervention group, but the inhibitory effect on mRNA and protein expression of IL-6 was stronger than that of bortezomil. Conclusion: sphingolipids inhibitor NB-DNJ can affect the expression of osteoclast activator in MM and the effect of sphingolipids inhibitor NB-DNJ on MBD is superior to that of bortezomil in vitro.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R733.3

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