miR-338-3p在肝癌组织中的临床意义和对肝癌细胞的影响

发布时间：2018-05-29 20:43

本文选题：肝细胞癌 + 微RNAs　；参考：《皖南医学院》2017年硕士论文

【摘要】：目的:探讨肝癌组织中mi RNA-338-3p基因表达的临床意义和该基因对肝癌细胞增殖、迁移、侵袭、上皮-间质转化的影响。方法:使用荧光实时定量PCR技术检测35对肝细胞癌和癌旁组织内mi RNA-338-3p基因表达情况,通过对比患者的一般临床资料,分析该基因的表达意义。通过携带目的基因的慢病毒感染体外培养的肝癌细胞HCCLM3和MHCC97-H,过表达肝癌细胞中的mi RNA-338-3p。再使用MTT实验、划痕实验、transwell实验、Western Bolt实验检测过表达基因对肝癌细胞的增殖、迁移、侵袭能力和上皮-间质转化相关蛋白的影响。结果:肝细胞癌组织中mi RNA-338-3p表达水平低于癌旁组织,并与患者肿瘤分化程度、癌栓、肿瘤直径、血管侵犯和术后生存期具有相关性(p0.05)。MTT实验:培养72小时后,实验组肝癌细胞HCCLM3和MHCC97-H的吸光度(1.153±0.232,1.247±0.247)低于阴性对照组(1.783±0.189,1.907±0.218)。划痕实验:HCCLM3和MHCC97-H细胞的实验组迁移距离分别为(89.7±30.7)μm和(164.7±33.5)μm,低于阴性对照组的(189.0±31.0)μm和(267.0±28.6)μm。Transwell实验:HCCLM3和MHCC97-H的实验组穿过膜细胞数为(35.3±12.2)个和(74.7±10.6)个,低于阴性对照组的(70.0±17.1)个和(120.7±22.6)个。Western Blot实验:空白对照组和阴性对照组的各蛋白表达无差异(p0.05),实验组的E-cad表达水平高于空白对照组和阴性对照组,而Vim表达水平低于另外两组。结论:肝癌组织内mi RNA-338-3p较癌旁组织呈低表达状态,低表达的mi RNA-338-3p患者较高表达者,预后更差、生存期更短。体外实验提示,高表达mi RNA-338-3p能抑制肝癌细胞的增殖能力,并可能通过上皮-间质转化的过程降低肝癌细胞迁移侵袭能力。
[Abstract]:Aim: to investigate the clinical significance of mi RNA-338-3p gene expression in hepatocellular carcinoma (HCC) and its effect on proliferation, migration, invasion and epithelial-interstitial transformation of HCC cells. Methods: the expression of mi RNA-338-3p gene in hepatocellular carcinoma and paracancerous tissues was detected by fluorescence real-time quantitative PCR. The significance of the gene expression was analyzed by comparing the clinical data of the patients. Human hepatoma cells HCCLM3 and MHCC97-H were infected by lentivirus carrying the target gene, which overexpressed mi RNA-338-3p. The effects of overexpression genes on proliferation, migration, invasion and epithelial-mesenchymal transformation of hepatoma cells were detected by MTT assay and Western Bolt assay. Results: the expression of mi RNA-338-3p in hepatocellular carcinoma was lower than that in adjacent tissues, and correlated with tumor differentiation, tumor thrombus, tumor diameter, vascular invasion and survival time. The absorbance of HCCLM3 and MHCC97-H in experimental group was 1.153 卤0.232 卤0.247 卤0.247), which was lower than that in negative control group (1.783 卤0.189). The migration distances of the two groups were 89.7 卤30.7 渭 m and 164.7 卤33.5 渭 m, respectively, which were lower than those of the negative control group (189.0 卤31.0) 渭 m and 267.0 卤28.6 渭 m.Transwell. The number of cells passing through the membrane of the two groups were 35.3 卤12.2 and 74.7 卤10.6, respectively. Western Blot test showed that there was no difference in protein expression between the blank control group and the negative control group. The expression level of E-cad in the experimental group was higher than that in the blank control group and the negative control group, but the Vim expression level was lower than that in the other two groups. Conclusion: the expression of mi RNA-338-3p in HCC is lower than that in paracancerous tissues. In patients with low expression of mi RNA-338-3p, the prognosis is worse and the survival time is shorter. The results showed that the high expression of mi RNA-338-3p could inhibit the proliferation of hepatoma cells and decrease the ability of migration and invasion of hepatoma cells through epithelial-interstitial transformation.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7

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