EMT相关指标TGF-β1、Slug及E-cadherin在胆管癌中的表达及意义

发布时间：2018-05-30 12:52

本文选题：胆管癌 + TGF-β1　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:探讨TGF-β1、Slug及E-cadherin在胆管癌中的表达及临床相关性,分析其在胆管癌发生及发展中的作用,为胆管癌的临床治疗提供实验依据。方法:选取2010-2016年河北医科大学第二医院70例有完整病理资料和临床资料的胆管癌组织蜡块标本,选取20例病理均证实为阴性的胆管癌癌旁组织蜡块标本作为阴性对照。采用免疫组化(S-P)法检测上述组织蜡块中TGF-β1、Slug及E-cadherin蛋白的表达情况,并分析其与患者的年龄、肿瘤分化程度、肿瘤大小、有无淋巴结转移、有无肝动脉或门静脉受侵等临床因素的关系。用SPSS21.0统计软件分析结果。结果:1 TGF-β1、Slug及E-cadherin在胆管癌组织中阳性表达率分别为67.1%、62.9%、41.4%,在癌旁正常胆管组织中的阳性表达率分别为25.0%、30.0%、80.0%,三种蛋白在胆管癌组织中和癌旁正常胆管组织中的表达差异均有统计学意义。2在胆管癌组织中,TGF-β1与Slug的阳性表达率在淋巴结转移组高于无淋巴结转移组(P0.05),在肝动脉或门静脉受侵组高于无受侵组(P0.05)。与病人年龄、肿瘤大小、肿瘤分化程度无相关性(P0.05)。E-cadherin的阳性表达率在淋巴结转移组低于无淋巴结转移组(P0.05),在肝动脉或门静脉受侵组低于无受侵组(P0.05)。E-cadherin的阳性表达与病人年龄、肿瘤大小、肿瘤分化程度无相关性(P0.05)。3在胆管癌组织中,TGF-β1与Slug的表达呈正相关(r=0.658,P0.05);TGF-β1与E-cadherin的表达呈负相关(r=-0.527,P0.05);Slug与E-cadherin的表达呈负相关(r=-0.526,P0.05)。结论:1与胆管癌旁正常腺体相比,TGF-β1、Slug在胆管癌中的表达明显升高,且其表达水平与胆管癌的淋巴结转移、肝动脉或门静脉受侵有关。提示二者在胆管癌发生、浸润、转移过程中起重要作用,可作为判断胆管癌临床进展及预后的重要指标。2与胆管癌旁正常腺体相比,E-cadherin在胆管癌中的表达明显降低,且其表达水平与胆管癌的淋巴结转移、肝动脉或门静脉受侵有关。提示其参与胆管癌发生、浸润、转移过程。3 E-cadherin表达的降低是EMT发生的标志性事件,TGF-β1与Slug在调控EMT过程中存在协同作用。EMT是胆管癌发生发展过程中的重要现象,为胆管癌的浸润转移机制提供一种合理解释。
[Abstract]:Objective: to investigate the expression of TGF- 尾 _ 1Slug and E-cadherin in cholangiocarcinoma and its clinical correlation, and to analyze the role of TGF- 尾 _ 1Slug and E-cadherin in the occurrence and development of cholangiocarcinoma, and to provide experimental evidence for the clinical treatment of cholangiocarcinoma. Methods: 70 paraffin specimens of cholangiocarcinoma tissues were collected from the second Hospital of Hebei Medical University from 2010 to 2016, and 20 samples of adjacent tissues with negative pathology were selected as negative control. The expression of TGF- 尾 1 Slug and E-cadherin protein in the wax mass was detected by immunohistochemical staining (S-P) method. The expression of TGF- 尾 1 Slug and E-cadherin protein was analyzed by immunohistochemical staining. The expression of TGF- 尾 1 Slug and E-cadherin protein was analyzed with age, tumor differentiation, tumor size and lymph node metastasis. Relationship between clinical factors such as hepatic artery or portal vein invasion. The results were analyzed by SPSS21.0 software. Results the positive expression rates of 1 TGF- 尾 1 Slug and E-cadherin in cholangiocarcinoma tissues were 67.1% and 62.9%, respectively. The positive expression rates in adjacent normal bile duct tissues were 25.010% and 80.0%, respectively. There were significant differences among the three proteins in cholangiocarcinoma tissues and adjacent normal bile duct tissues. The positive rates of TGF- 尾 1 and Slug in cholangiocarcinoma were higher in lymph node metastasis group than in non-lymph node metastasis group, and higher in hepatic artery or portal vein invasion group than in non-invasive group. The positive expression rate of P0.05U. E-cadherin in lymph node metastasis group was lower than that in non-lymph node metastasis group, and the positive expression rate in hepatic artery or portal vein group was lower than that in non-invading group, and the positive rate of E-cadherin expression was lower in hepatic artery or portal vein group than that in non-invasive group. There was a positive correlation between the expression of TGF- 尾 1 and Slug in cholangiocarcinoma tissues. There was a negative correlation between the expression of TGF- 尾 1 and E-cadherin. There was a negative correlation between the expression of TGF- 尾 1 and E-cadherin. Conclusion the expression of TGF- 尾 1 Slug in cholangiocarcinoma is significantly higher than that in adjacent normal glands. The expression level of TGF- 尾 1 Slug is related to lymph node metastasis, hepatic artery or portal vein invasion of cholangiocarcinoma. These results suggest that the expression of E-cadherin in cholangiocarcinoma is significantly lower than that in the normal gland adjacent to cholangiocarcinoma, and it may play an important role in the development, invasion and metastasis of cholangiocarcinoma. It can be used as an important index to judge the clinical progress and prognosis of cholangiocarcinoma. The expression level was related to lymph node metastasis, hepatic artery or portal vein invasion of cholangiocarcinoma. It is suggested that the decrease of E-cadherin expression in the process of carcinogenesis, invasion and metastasis of cholangiocarcinoma is a landmark event of EMT. TGF- 尾 1 and Slug have synergistic effect on the regulation of EMT. EMT is an important phenomenon in the process of occurrence and development of cholangiocarcinoma. It provides a reasonable explanation for the mechanism of invasion and metastasis of cholangiocarcinoma.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.8

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