IL-17基因多态性与胃癌易感性的相关性研究

发布时间：2018-05-31 08:34

本文选题：胃肿瘤 + 白介素-17(IL-17)　；参考：《青岛大学》2017年硕士论文

【摘要】：研究目的研究胃癌发生的分子遗传机制,为胃癌的预防、胃癌高危人群的筛查、诊断提供依据,也能对胃癌的治疗提供新的思路。探讨IL-17基因rs2275913、rs763780、rs3748067位点多态性与胃癌易感性的相关性。研究方法采取病例对照研究,收集2014年12月—2015年12月青岛市市立医院355例汉族胃癌患者组成胃癌组,选取同期在体检中心进行健康体检的300名正常者组成对照组。两组人群均为汉族人口。采集两组患者外周血液,参照血液基因组DNA提取试剂盒(天根生化科技有限公司)的说明书提取全血基因组DNA,PCR扩增目的基因片段,采用DNA直接测序法检测IL-17基因rs2275913、rs763780、rs3748067位点基因型,探讨其位点多态性与胃癌发病风险之间的关系。结果胃癌组与对照组IL-17基因rs2275913位点基因型分布差异有统计学意义(cc2=17.192,P=0.000);与GG基因型相比,携带AA基因型的个体胃癌发病风险增加,差异有统计学意义(c2=16.829,P0.05;OR=2.891,95%CI=1.721-4.857);携带GA基因型的个体胃癌发病风险增加(OR=1.172,95%CI=0.841-1.634),但差异无统计学意义(c2=0.878,P0.05)。与携带rs763780位点CC基因型相比,携带CT基因型的个体胃癌易感性增高(OR=1.132,95%CI=0.810-1.583),但是差异没有统计学意义(c2=0.526,P0.05),携带TT基因型的个体胃癌易感性增高(OR=1.404,95%CI=0.755-2.609),差异也没有统计学意义(c2=1.156,P0.05)。与携带rs3748067位点TT基因型相比,携带CT基因型的个体胃癌易感性增高(OR=1.402,95%CI=0.748-1.451),但是差异没有统计学意义(c2=0.059,P0.05),携带CC基因型的个体胃癌易感性降低(OR=0.661,95%CI=0.408-1.072),差异也没有统计学意义(c2=2.830,P0.05)。对于rs2275913位点,H.Pylori阳性组AA基因型频率明显高于H.Pylori阴性组(18.2%比8.5%),差异有统计学意义(P0.05);而H.Pylori阳性组GA、GG基因型频率与H.Pylori阴性组相比,差异无统计学意(P0.05)。对于rs3748067位点,H.Pylori阳性组与H.Pylori阴性组基因型分布无明显差异(P0.05)。结论IL-17基因rs2275913 A等位基因的携带会增加胃癌发病风险,IL-17基因rs763780、rs3748067位点多态性与胃癌发病风险无明显相关性。
[Abstract]:Objective to study the molecular genetic mechanism of gastric cancer, to provide evidence for the prevention of gastric cancer, screening and diagnosis of high risk population of gastric cancer, and to provide new ideas for the treatment of gastric cancer. To investigate the association between the polymorphism of rs2275913, rs763780, rs3748067 and the susceptibility of gastric cancer. Methods A case-control study was conducted to collect 355 patients with gastric cancer of Han nationality from December 2014 to December 2015 in Qingdao City Hospital. Both groups were Han population. The peripheral blood fluid of two groups of patients was collected and the target gene fragment was amplified by PCR with reference to the instructions of the blood genomic DNA extraction kit. The genotypes of IL-17 gene rs2275913, rs763780, rs3748067 were detected by DNA direct sequencing. To investigate the relationship between locus polymorphism and the risk of gastric cancer. Results the genotype distribution of rs2275913 locus of IL-17 gene in gastric cancer group was significantly different from that in control group, and the risk of gastric cancer was increased in individuals with AA genotype compared with GG genotype. The difference was statistically significant (P < 0.05). The risk of gastric cancer in individuals with GA genotype increased by 1.172 ~ 95CIN 0.841-1.634, but there was no significant difference between 0.878P0.05and 0.878P0.05.The difference was statistically significant (P < 0.05), but there was no significant difference in the risk of gastric cancer in individuals with GA genotype (P = 0.878P0.05), but there was no significant difference in the risk of gastric cancer in individuals with GA genotype (P < 0.05), but there was no significant difference between the two groups. Compared with CC genotype carrying rs763780 locus, the susceptibility of individuals with CT genotype was higher than that of individuals with CT genotype. The susceptibility of individuals with CT genotype was 0.810-1.583, but the difference was not statistically significant, but there was no significant difference in the susceptibility of individuals with TT genotype. The susceptibility of individuals with TT genotype was 0.755-2.609, and the difference was not statistically significant. Compared with TT genotype carrying rs3748067 locus, the susceptibility of individuals carrying CT genotype to gastric cancer was increased. The susceptibility of individuals with CT genotype was 0.748-1.451C, but the difference was not statistically significant. The susceptibility of individuals with CC genotype was lower than that of individuals with rs3748067 locus TT genotype (CI 0.408-1.072), and there was no significant difference between them. The frequency of AA genotype in rs2275913 positive group was significantly higher than that in H.Pylori negative group (18.2% vs 8.5%), the difference was statistically significant (P 0.05), but there was no significant difference between H.Pylori positive group and H.Pylori negative group. There was no significant difference in genotype distribution between H.Pylori positive group and H.Pylori negative group for rs3748067 locus. Conclusion carrying the rs2275913 A allele of IL-17 gene may increase the risk of gastric cancer. The polymorphism of rs763780 rs3748067 in IL-17 gene has no significant correlation with the risk of gastric cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

【参考文献】