CDCA8和INCENP mRNA在肝细胞癌组织中的表达及其临床意义

发布时间：2018-06-01 00:22

本文选题：肝细胞癌 + 肿瘤基因组图谱　；参考：《中国肿瘤生物治疗杂志》2017年02期

【摘要】：目的:探讨CDCA8和INCENP mRNA在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及其临床意义。方法:使用Illumina Nextbio芯片数据库分析原发性HCC组织和相应癌旁组织中CDCA8和INCENP mRNA表达情况,在癌症基因组图谱(The Cancer Genome Atlas,TCGA)肝癌数据库中使用RNA-Seq mRNA表达数据进行验证,并结合TCGA临床数据分析CDCA8和INCENP mRNA表达水平与HCC患者临床病理特征及预后的关系,使用基因集富集软件分析目的基因高表达的相关通路。结果:CDCA8和INCENP mRNA在HCC组织中表达水平显著上调(P0.01)。CDCA8 mRNA表达水平与HCC的组织学分级、瘤体大小、肿瘤复发、美国东部肿瘤协作组织(ECOG)评级、血清AFP水平、肿瘤基因拷贝数变异显著相关(P0.05);INCENP mRNA表达水平与HCC的组织学分级、肿瘤复发、ECOG评级、血清AFP水平、肿瘤基因突变总数和肿瘤基因拷贝数变异显著相关(P0.05)。CDCA8高表达组患者的OS及TFS显著低于CDCA8低表达组患者(P0.01);INCENP高表达组患者的OS显著低于INCENP低表达组患者(P0.05),而TFS无显著差异(P0.05)。多因素分析显示,CDCA8 mRNA水平是预测HCC患者不良预后的独立因素(P0.01)。基因集富集分析表明,CDCA8和INCENP可能在细胞周期之外的其他生物学信号通路中发挥一定作用。结论:CDCA8和INCENP mRNA在HCC组织中呈现显著高表达。CDCA8可能成为HCC预后的独立风险因素和新的治疗靶点。
[Abstract]:Objective: to investigate the expression and clinical significance of CDCA8 and INCENP mRNA in hepatocellular carcinoma. Methods: Illumina Nextbio microarray database was used to analyze the expression of CDCA8 and INCENP mRNA in primary HCC tissues and corresponding adjacent tissues, and RNA-Seq mRNA expression data were used to verify the expression of CDCA8 and INCENP mRNA in the cancer genome map of the Cancer Genome Atlas TCGA. Based on the clinical data of TCGA, the relationship between the expression of CDCA8 and INCENP mRNA and the clinicopathological characteristics and prognosis of HCC patients was analyzed. The gene set enrichment software was used to analyze the related pathway of high expression of target gene. Results the expression levels of INCENP mRNA and CDCA8 were significantly up-regulated in HCC tissues. The expression levels of mRNA and HCC were correlated with the histological grade, tumor size, tumor recurrence, Ecog rating and serum AFP level. There was a significant correlation between the expression of INCENP mRNA and the histological grade of HCC, the grade of ECOG and the level of serum AFP. There was a significant correlation between the total mutation of tumor gene and the variation of the copy number of tumor gene. The OS and TFS of the patients with high expression of P0.05N. CDCA8 were significantly lower than those of the patients with high expression of P0.01INCENP and the patients with high expression of INCENP, but there was no significant difference in TFS between the patients with high expression and the patients with low expression of INCENP. Multivariate analysis showed that CDCA8 mRNA level was an independent predictor of poor prognosis in patients with HCC. Gene set enrichment analysis showed that CDCA8 and INCENP may play a role in other biological signaling pathways outside the cell cycle. Conclusion the high expression of CDCA8 and INCENP mRNA in HCC may be an independent risk factor and a new therapeutic target for the prognosis of HCC.
【作者单位】：复旦大学生命科学学院遗传工程国家重点实验室;
【基金】：国家科技重大专项资助项目(No.2013ZX10002010)~~
【分类号】：R735.7

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