DDX3、uPAR在结直肠癌及其肝转移中的表达及相关性分析

发布时间：2018-06-01 02:09

  本文选题：DDX3 + uPAR　； 参考：《川北医学院》2017年硕士论文

【摘要】：目的:通过检测CRC及其肝转移中DEAD-box多肽3(DDX3)及尿激酶型纤溶酶激活剂受体(uPAR)表达情况,探究DDX3和uPAR在CRC及其肝脏转移中表达及其与CRC患者临床病理特征(性别、年龄、肿瘤部位、分化类型、CEA、肿瘤大小、浸润深度、TNM分期、淋巴结转移、肝转移、)之间的关系。为进一步研究DDX3、uPAR在CRC发生、发展、远处转移尤其是肝转移中的作用提供有用线索。方法:收集川北医学院附属医院2013年01月至2015年06月CRC患者术后标本60例,含肝转移标本14例;CRC癌旁正常组织标本60例,CRC肝转移癌旁正常组织标本14例。采用免疫组化方法检测标本中DDX3、uPAR表达,并进一步分析DDX3、uPAR在CRC及其肝转移病理组织中的表达及其与CRC患者的临床病理特征的相互关系。所有数据均采用IBM SPSS Statistics 21.0软件分析。结果:DDX3在CRC标本中阳性表达率63.33%,在CRC癌旁正常组织标本中阳性表达率20.00%,两者差异有统计学意义(X~2=21.429,P0.05);在CRC肝转移标本中阳性表达率92.86%,在CRC肝转移癌旁正常组织标本中阳性表达率14.29%,两者差异有统计学意义(X~2=14.359,P0.05)。uPAR在CRC标本中阳性表达率68.33%,在CRC癌旁正常组织标本中阳性表达率23.33%,两者差异有统计学意义(X~2=22.691,P0.05);在CRC肝转移标本中阳性表达率100.00%,在CRC肝转移癌旁正常组织标本中阳性表达率7.14%,两者差异有统计学意义(X~2=20.677,P0.05)。DDX3的表达在CRC患者的性别(X~2=0.027,P0.05)、年龄(X~2=0.000,P0.05)、肿瘤部位(X~2=0.058,P0.05)组间差异无统计学意义;而在分化类型(X~2=5.021,P0.05)、CEA量(X~2=3.305,P0.05)、肿瘤大小(X~2=3.326,P0.05)、浸润深度(X~2=9.569,P0.05)、TNM分期(X~2=14.712,P0.05)、淋巴结转移(X~2=10.811,P0.05)、肝转移(X~2=5.893,P0.05)组间差异有统计学意义。uPAR的表达在CRC患者的性别(X~2=0.000,P0.05)、年龄(X~2=0.000,P0.05)、肿瘤部位(X~2=0.094,P0.05)组间差异无统计学意义;而在分化类型(X~2=3.371,P0.05)、CEA(X~2=6.133,P0.05)肿瘤大小(X~2=6.318,P0.05)、浸润深度(X~2=9.569,P0.05)、TNM分期(X~2=15.561,P0.05)、淋巴结转移(X~2=8.594,P0.05)、肝转移(X~2=6.661,P0.05)组间差异有统计学意义。DDX3和uPAR均在CRC标本中表达,60例CRC标本中共同表达33例,阳性共表达率55.00%,采用Spearman相关分析得:(r=-0.523,-0.8r≤-0.5;P0.05),两者呈负相关,有统计学意义。结论:DDX3、uPAR与CRC的发生、发展、和临床预后密切相关,二者还可能共同参与了CRC的局部侵袭和远处转移过程,并在CRC的发生、发展和远处转移过程中相互协同、共同促进,但其与CRC局部侵润和分化过程,远处转移尤其是异时性肝脏转移的关系还有待于进一步实验研究去证实。这些特征有助于DDX3、uPAR成为临床诊断和治疗CRC及其肝转移患者新的检测因子和治疗靶点,而同时研究DDX3和uPAR将更加有利于CRC及其肝转移患者的临床检测诊断、分子靶向治疗和疾病预后判断。
[Abstract]:Objective: to investigate the expression of DDX3 and uPAR in CRC and its liver metastasis and its clinicopathological features (sex, age, tumor location) by detecting the expression of DEAD-box polypeptide 3ddx3 and urokinase type plasminogen activator receptor (UPAR) in CRC and its liver metastasis. The relationship between tumor size, TNM stage, lymph node metastasis and liver metastasis. It provides useful clues for further study on the role of DDX3 upar in the pathogenesis, development and distant metastasis of CRC, especially in liver metastasis. Methods: from January 2013 to June 2015, 60 patients with CRC and 14 patients with liver metastasis were collected from affiliated Hospital of North Sichuan Medical College. The expression of DDX3 upar was detected by immunohistochemical method. The expression of DDX3 upar in CRC and its liver metastases and their relationship with the clinicopathological features of CRC patients were analyzed. All the data were analyzed by IBM SPSS Statistics 21. 0 software. Results the positive expression rate of CRC was 63.33 in CRC and 20.00 in normal tissues adjacent to CRC. The difference between them was statistically significant (P 0.05), and 92.86 in CRC liver metastases, 92.86 in normal tissues adjacent to CRC liver metastasis. The positive expression rate was 14.29. The difference between the two groups was statistically significant. The positive expression rate of upar was 68.33 in CRC and 23.3333 in normal tissues adjacent to CRC. The difference was statistically significant between the two groups. The positive expression rate of upar in CRC liver metastases was 100.005. The positive expression rate was 7.14 in normal tissues adjacent to CRC liver metastases. The difference between the two groups was statistically significant. There was no significant difference between the two groups in the sex of CRC patients. 鑰屽湪鍒嗗寲绫诲瀷(X~2=5.021,P0.05),CEA閲，

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