HCRP1基因在胃癌中的表达及与胃癌临床病理特征的相关性研究

发布时间：2018-06-01 23:23

本文选题：胃癌 + HCRP1基因　；参考：《济南大学》2017年硕士论文

【摘要】：目的研究胃癌组织中HCRP1基因的表达情况,并结合所收集患者的临床病理特征及相关资料,分析HCRP1基因的不同表达与患者临床病理特征之间的相关性,以及HCRP1基因的不同表达对胃癌患者生存预后的影响。方法收集胃癌病人手术切除的癌旁正常组织及胃癌组织,应用免疫组织化学染色方法检测所有癌旁正常组织及胃癌组织中HCRP1蛋白的表达情况,应用逆转录一聚合酶链式反应(RT-PCR)方法检测所有癌旁正常组织及胃癌组织中HCRP1mRNA的表达情况。并应用单因素方差分析HCRP1基因表达的影响因素。同时应用Kaplan-Meier生存分析法分析HCRP1蛋白的不同表达与患者远期生存的关系,COX回归模型分析患者预后的独立影响因素。结果免疫组化染色结果显示HCRP1蛋白在癌旁正常胃粘膜组织中的表达量要高于相邻胃癌组织中的表达量,且差异具有统计学意义,但HCRP1蛋白在不同分化程度胃癌组织中的表达差异无统计学意义。单因素方差分析发现HCRP1蛋白的不同表达与患者的淋巴结转移、远处转移、TNM分期以及HCRP1蛋白表达有关。RT-PCR结果显示HCRP1mRNA在癌旁正常胃粘膜组织中高表达,而在胃癌组织中呈现低表达,差异具有统计学意义。并且通过RT-PCR检测发现不同分化程度的胃癌组织中HCRP1表达量不同,但差异无统计学意义。Kaplan-Meier生存分析显示HCRP1蛋白高表达的胃癌患者其远期生存率要远远高于HCRP1蛋白低表达的胃癌患者(P0.05),单因素生存分析发现HCRP1蛋白表达、淋巴结转移、远处转移及TNM分期是预后的影响因素。多因素COX分析发现远处转移和TNM分期是预后的独立影响因素。结论HCRP1基因可以抑制胃癌的生长及增值,其在癌旁正常胃粘膜组织中高表达,在胃癌组织中低表达。HCRP1基因表达与胃癌患者是否有淋巴结转移、远处转移以及TNM分期有关,与患者的年龄、性别、肿块大小和分化程度无关。并且HCRP1蛋白高表达的胃癌患者其预后要好于HCRP1蛋白低表达的胃癌患者。因此可将HCRP1基因作为一种预测胃癌患者术后生存的指标应用于临床,同时对于评估胃癌的临床分期和指导其治疗也有重要的意义。
[Abstract]:Objective to study the expression of HCRP1 gene in gastric cancer and analyze the correlation between the expression of HCRP1 gene and the clinicopathological features of gastric cancer. And the effect of different expression of HCRP1 gene on survival and prognosis of gastric cancer patients. Methods the expression of HCRP1 protein was detected by immunohistochemical staining in normal tissues and gastric cancer tissues of patients with gastric cancer. Reverse transcription-polymerase chain reaction (RT PCR) was used to detect the expression of HCRP1mRNA in all adjacent normal tissues and gastric cancer tissues. Univariate ANOVA was used to analyze the influencing factors of HCRP1 gene expression. At the same time, Kaplan-Meier survival analysis was used to analyze the relationship between the expression of HCRP1 protein and the long-term survival of patients. The Cox regression model was used to analyze the independent prognostic factors of patients. Results Immunohistochemical staining showed that the expression of HCRP1 protein in adjacent normal gastric mucosa was higher than that in adjacent gastric cancer tissues, and the difference was statistically significant. However, there was no significant difference in the expression of HCRP1 protein in different differentiated gastric cancer tissues. Univariate ANOVA analysis showed that different expression of HCRP1 protein was related to lymph node metastasis, distant metastasis stage and HCRP1 protein expression. RT-PCR results showed that HCRP1mRNA was highly expressed in adjacent normal gastric mucosa, but low in gastric cancer tissue. The difference is statistically significant. The expression of HCRP1 was found to be different in gastric cancer tissues with different differentiation degree by RT-PCR detection. However, Kaplan-Meier survival analysis showed that the long-term survival rate of patients with high expression of HCRP1 protein was much higher than that of patients with low expression of HCRP1 protein. Univariate survival analysis showed the expression of HCRP1 protein and lymph node metastasis. Distant metastasis and TNM stage were the prognostic factors. Multivariate COX analysis showed that distant metastasis and TNM staging were independent prognostic factors. Conclusion HCRP1 gene can inhibit the growth and proliferation of gastric cancer. It is highly expressed in normal gastric mucosa adjacent to gastric cancer. The low expression of .HCRP1 gene in gastric cancer is related to lymph node metastasis, distant metastasis and TNM staging. There was no correlation between age, sex, tumor size and differentiation. The prognosis of gastric cancer patients with high expression of HCRP1 protein was better than that with low expression of HCRP1 protein. Therefore, HCRP1 gene can be used as a predictor of postoperative survival in patients with gastric cancer. It is also of great significance to evaluate the clinical stage and guide the treatment of gastric cancer.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

【参考文献】