循环微粒对口腔癌进展的作用机制研究

发布时间：2018-06-01 23:40

本文选题：循环微囊泡 + 口腔癌　；参考：《中国现代医学杂志》2017年21期

【摘要】：目的比较正常人和口腔鳞状细胞癌患者循环微粒的含量,并探究循环微粒在口腔癌进展中的作用。方法差速离心法收集正常人和口腔癌循环微粒。流式细胞术检测循环微粒的浓度;MTT法和Ed U实验检测细胞增殖活性,划痕实验和Transwell实验分别检测细胞迁移和侵袭能力;裸鼠移植瘤模型检测循环微粒对肿瘤细胞体内成瘤的影响。结果口腔癌患者循环微粒的含量比正常人的高,且循环微粒的浓度与口腔癌患者血液高凝状态密切相关。口腔癌患者来源的循环微粒促进口腔癌细胞增殖、迁移、侵袭和体内成瘤。结论口腔癌患者来源的循环微粒促进口腔癌细胞增殖、迁移、侵袭和体内成瘤,在口腔癌的发生、发展中起重要作用。
[Abstract]:Objective to compare the content of circulating particles in normal subjects and patients with oral squamous cell carcinoma (OSCC) and to explore the role of circulating particles in the progression of oral squamous cell carcinoma (OSCC). Methods the circulating particles of normal and oral cancer were collected by differential centrifugation. Flow cytometry was used to detect the concentration of circulating particles. MTT assay and Ed U assay were used to detect the cell proliferation activity. The cell migration and invasion ability were detected by scratch assay and Transwell assay respectively. The effect of circulating particles on tumorigenesis of tumor cells in nude mice was examined. Results the content of circulating particles in patients with oral cancer was higher than that in normal controls, and the concentration of circulating particles was closely related to the hypercoagulability of blood in patients with oral cancer. Circulating particles from patients with oral cancer promote the proliferation, migration, invasion and tumorigenesis of oral cancer cells. Conclusion the circulating particles from patients with oral cancer promote the proliferation, migration, invasion and tumorigenesis of oral cancer cells, which play an important role in the occurrence and development of oral cancer.
【作者单位】：苏州大学附属第一医院口腔科;
【分类号】：R739.8

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