双靶向HGFK1溶瘤腺病毒对裸鼠人胃癌移植瘤抑制作用的研究

发布时间：2018-06-02 07:15

本文选题：HGFK1 + 溶瘤腺病毒　；参考：《青岛大学》2017年硕士论文

【摘要】：目的研究双靶向肝细胞生长因子第一环状结构域基因(HGFK1)溶瘤腺病毒对裸鼠人胃癌移植瘤的抑制杀伤作用。方法向裸鼠右侧前腋下注射人胃癌细胞SGC7901,建立人胃癌裸鼠移植瘤模型。并分别瘤内注射双靶向HGFK1溶瘤腺病毒(TH-AD-HGFK1),双靶向溶瘤腺病毒(TH-AD),野生型溶瘤腺病毒(AD5)和PBS,观察移植瘤的生长情况和裸鼠胃癌模型的生存时间。结果成功建立裸鼠胃癌移植瘤模型;试验后28天试验组(TH-AD-HGFK1)的裸鼠移植瘤体积为1563.61±294.47mm3和,TH-AD组为2674.55±408.92mm3,AD5组为2792.94±533.57mm3,PBS组为3372.22±421.26mm3,试验组移植瘤体积与各组相比的P值分别为P=0.029,P=0.020,P=0.004,差异有统计学意义(P0.05);处死裸鼠剥离移植瘤后称重发现试验组移植瘤重量为3.31±0.44g,TH-AD组为3.59±0.34g,AD5组为3.66±0.41g,PBS组为3.91±0.48g,试验组瘤重各组相比的P值分别为P=0.239,P=0.140,P=0.013,试验组瘤重与PBS组相比明显减轻(P0.05),其余各组之间则无明显差异(P0.05)。观察至试验后78天试验组裸鼠全部存活(100%),TH-AD组和AD5组裸鼠存活率均为60%,PBS组裸鼠全部死亡(0%)。结论双靶向HGFK1溶瘤腺病毒对裸鼠人胃癌移植瘤具有抑制杀伤作用,并可延长荷瘤裸鼠的生存时间。
[Abstract]:Objective to study the inhibitory effect of double targeting hepatocyte growth factor (HGFK1) oncogene HGFK1 on xenografts of human gastric cancer in nude mice. Methods Human gastric cancer cell line SGC7901 was injected into the right anterior axillary of nude mice to establish transplanted tumor model of human gastric cancer. TH-AD-HGFK1, TH-AD5 and AD5 were injected into the tumor respectively to observe the growth of transplanted tumor and the survival time of gastric cancer model in nude mice. Results the transplanted tumor model of gastric cancer was successfully established in nude mice. 28 days after the trial, the volume of transplanted tumor of nude mice in test group was 1563.61 卤294.47mm3 and 2674.55 卤408.92 mm3AD5 in TH-AD group was 3372.22 卤421.26mm3 in TH-AD group and 2792.94 卤533.57mm3m3pBS group in TH-AD group. The volume of transplanted tumor in test group was 3372.22 卤421.26mm3 compared with each group (P = 0.029, P = 0.020, P = 0.004, respectively), the difference was statistically significant (P 0.05). It was found that the weight of transplanted tumor was 3.31 卤0.44 g / g TH-AD group (3.59 卤0.34 g / d), 3.66 卤0.41 g / g PBS group (3.91 卤0.48 g / d), respectively (P < 0.05). The tumor weight of the test group was 0.239g / g ~ 0.140g / g TH-AD group (P < 0.05), but there was no significant difference between the other groups and the PBS group (P < 0.05). It was observed that the survival rate of all the nude mice in the experimental group was 60% and that in the AD5 group was 60%. The survival rate of all the nude mice in the test group was 100% and 100% respectively, and the survival rate of all the nude mice in the AD5 group was 100% (P < 0.05). Conclusion double targeting HGFK1 adenovirus can inhibit the growth of human gastric cancer transplanted tumor in nude mice and prolong the survival time of nude mice.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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