环孢素A对人胃癌BGC823细胞增殖与凋亡的影响

发布时间：2018-06-03 10:27

本文选题：胃肿瘤 + 环孢素A　；参考：《安徽医科大学学报》2017年11期

【摘要】：目的研究环孢素A(Cs A)对人胃癌BGC823细胞的生长增殖以及凋亡的影响。方法 MTT法检测不同浓度Cs A处理24、48、72 h后对BGC823细胞增殖抑制率;流式细胞术(FCM)检测细胞周期、活性氧(ROS)含量及细胞线粒体膜电位(△Ψm)改变;Annexin-V-FITC/PI双染法检测细胞凋亡率。结果 Cs A在5~20μmol/L浓度范围内和24~72 h时间范围对BGC823细胞增殖有显著抑制作用,与药物剂量、作用时间呈现量效和时效的依赖性;FCM结果显示Cs A浓度依耐性使细胞周期阻滞于G0/G1期,与对照组相比差异有统计学意义(F=33.45,P0.05,P0.01);细胞凋亡率随Cs A作用浓度增加而增加;5~20μmol/L Cs A浓度范围细胞内ROS含量显著增加(F=46.17,P0.01),细胞线粒体膜电位明显降低。结论 Cs A对BGC823细胞有抑制增殖和诱导凋亡作用,其机制可能与阻滞细胞生长周期、细胞内ROS含量增加以及线粒体膜电位下降相关。
[Abstract]:Objective to study the effects of cyclosporine (A(Cs A) on the growth, proliferation and apoptosis of human gastric cancer BGC823 cells. Methods MTT assay was used to detect the proliferation inhibition rate of BGC823 cells treated with different concentrations of Cs A for 72 h, and flow cytometry was used to detect cell cycle, reactive oxygen species (Ros) content and mitochondrial membrane potential (蠄 m). The apoptosis rate was detected by Annexin-V-FITC / Pi double staining. Results Cs A significantly inhibited the proliferation of BGC823 cells in the concentration range of 5 渭 mol/L (20 渭 mol/L) and 24 h for 72 h. The results showed that the concentration of Cs A was dependent on dose, time and time of action. The results showed that the cell cycle was blocked in the G0/G1 phase by the concentration of CSA in accordance with tolerance. Compared with the control group, the cell apoptosis rate increased with the increase of Cs A concentration, and the ROS content increased significantly in the range of 20 渭 mol/L Cs A concentration, and the cell mitochondrial membrane potential was significantly lower than that in the control group (P < 0.05), and the cell apoptosis rate was significantly higher than that in the control group (P < 0.05), and the cell apoptosis rate increased with the increase of the concentration of Cs A, and the cell ROS content increased significantly in the range of 20 渭 mol/L Cs A concentration. Conclusion Cs A can inhibit the proliferation and induce apoptosis of BGC823 cells, and its mechanism may be related to the arrest of cell cycle, the increase of ROS content and the decrease of mitochondrial membrane potential.
【作者单位】：苏州市立医院北区消化科;安徽医科大学第一附属医院科研处;安徽医科大学第一附属医院消化内科;
【基金】：安徽省自然科学基金青年科学基金项目(编号:11040606Q24)
【分类号】：R735.2

【相似文献】