IER3基因在原发性肝癌中的表达及意义

发布时间：2018-06-05 10:12

本文选题：肝癌 + IER3　；参考：《青岛大学》2015年博士论文

【摘要】：研究背景:原发性肝癌是世界上最常见的恶性肿瘤之一,近年研究发现基因在肿瘤的发生、发展中具有重要作用。目前对基因在原发性肝癌中的表达及意义尚不明确。目的:1.从蛋白和基因两个水平上进行检测,明确IER3基因在原发性肝癌组织中的表达特点。2.明确IER3基因与临床病理特征及预后之间的关系。3.初步探讨IER3基因的作用机制。方法:1.收集淄博市中心医院2011年1月至2014年3月62例原发性肝癌组织及对应癌旁组织的石蜡标本,采用免疫组化法检测IER3蛋白、P53、ki-67、EGFR、AFP、NF-ΚB、XIAP、Nrf2、HO-1的表达情况。2.采用Real-Time PCR法检测不同组织中IER3基因的表达情况。3.IER3基因表达与临床病理特征及预后的关系。4.IER3基因表达与NF-ΚB、XIAP、Nrf2、HO-1的表达之间的关系。结果:1.IER3基因在原发性肝癌组织中的表达高于癌旁组织。2.IER3的表达与P53、ki-67、EGFR、肿瘤最大径及分化级别有相关性。3.IER3基因在原发性肝癌组织中的表达与患者PFS及OS相关。是无疾病生存期和疾病总生存期的独立危险因素。4.IER3基因表达与NF-ΚB、XIAP、Nrf2、HO-1的表达均相关,提示IER3基因可能通过NF-ΚB/XIAP通路及Nrf2/HO-1通路发挥作用。结论:IER3基因在原发性肝癌组织中的表达高于癌旁组织,IER3基因高表达提示肿瘤恶性度高,进展快,预后差。IER3是原发性肝癌一个有重要价值的标志物,是无疾病生存期和疾病总生存期的独立危险因素。可以预测患者的预后。IER3基因能够通过NF-ΚB/XIAP通路及Nrf2/HO-1通路发挥作用。
[Abstract]:Background: primary liver cancer is one of the most common malignant tumors in the world. At present, the expression and significance of gene in primary liver cancer are not clear. Purpose 1. The expression characteristics of IER3 gene in primary liver cancer tissues were determined from protein and gene levels. 2. To determine the relationship between IER3 gene and clinicopathological features and prognosis. To explore the mechanism of IER3 gene. Method 1: 1. From January 2011 to March 2014, 62 paraffin wax specimens of primary liver cancer and corresponding adjacent tissues were collected from central hospital of Zibo City. The expression of IER3 protein P53 ki-67 IER3 protein was detected by immunohistochemical method. The relationship between the expression of IER3 gene and the clinicopathological characteristics and prognosis was detected by Real-Time PCR method. 4. The relationship between the expression of IER3 gene and the expression of NF- K BX IAPP Nrf2nrf2HO-1.The relationship between the expression of IER3 gene and the clinicopathological characteristics and prognosis was analyzed by Real-Time PCR method. Results 1. The expression of IER3 gene in HCC was higher than that in paracancerous tissue. 2. The expression of IER3 was correlated with P53ki-67EGFR.3. the expression of IER3 gene in HCC was correlated with PFS and OS. 4. The expression of IER3 gene was correlated with the expression of NF- K BX XIAPN Nrf2HO-HO-1, suggesting that the IER3 gene may play a role through NF- B/XIAP pathway and Nrf2/HO-1 pathway. 4. The expression of NF- K gene is a risk factor of disease free survival and total disease survival. 4. The expression of IER3 gene is related to the expression of NF- K B/XIAP pathway and Nrf2/HO-1 pathway. Conclusion the high expression of 1% IER3 gene in primary liver cancer is higher than that in adjacent tissues. It suggests that the tumor has a high degree of malignancy, rapid progression and poor prognosis. IER3 is an important marker of primary liver cancer. It is an independent risk factor for disease free survival and total disease survival. It can predict the prognosis of patients. IER3 gene can play a role through NF- K B/XIAP pathway and Nrf2/HO-1 pathway.
【学位授予单位】：青岛大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R735.7

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