胃癌组织原钙黏蛋白10mRNA表达水平与预后的相关性

发布时间：2018-06-07 11:28

本文选题：胃癌 + 原钙黏蛋白　；参考：《中国癌症杂志》2017年01期

【摘要】：背景与目的:编码原钙黏蛋白10(protocadherin-10,PCDH10)的PCDH10基因启动子甲基化与胃癌患者不良预后相关。但PCDH10表达水平与胃癌预后的关系不明确。该研究旨在分析PCDH10表达水平与胃癌预后及临床病理因素间的关系,寻找预测胃癌患者复发及死亡风险的指标。方法:采用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR)方法检测115对胃癌组织与相应癌旁组织PCDH10 m RNA的表达水平,分析PCDH10 m RNA的表达水平与预后及临床病理因素的关系。采用Logistic回归分析建立预测患者5年内复发或死亡风险的模型。结果:PCDH10 m RNA低表达组与非低表达组相比,无进展生存时间(progression-free survival,PFS)与总生存时间(overall survival,OS)显著延长(P值分别为0.046与0.033),淋巴结转移较少(P=0.001),TNM分期较早(P=0.001)。Cox单因素分析发现,Lauren分型、T分期、N分期、M分期及PCDH10 m RNA表达水平与PFS及OS显著相关。包含PCDH10作为参数的Logistic回归模型对胃癌患者术后5年内复发或死亡风险的预测效率与仅包含传统临床病理学参数的Logistic回归模型的预测效率相当。结论:PCDH10低表达胃癌患者淋巴结转移较少,TNM分期较早,预后较好,可以作为预测胃癌患者预后的辅助指标。基于PCDH10表达水平的Logistic回归模型可以在淋巴结转移情况不明时起到辅助判断患者预后的作用。
[Abstract]:Background & AIM: methylation of PCDH10 promoter encoding procadherin-10PCDH10 is associated with poor prognosis in patients with gastric cancer. However, the relationship between the expression of PCDH10 and the prognosis of gastric cancer is not clear. The purpose of this study was to analyze the relationship between the expression of PCDH10 and prognosis and clinicopathological factors of gastric cancer, and to find out the predictors of recurrence and death risk of gastric cancer patients. Methods: the expression of PCDH10 m RNA in gastric cancer tissues and adjacent tissues was detected by real-time fluorescent quantitative polymerase chain reaction- PCR. The relationship between the expression of PCDH10 m RNA and prognosis and clinicopathological factors was analyzed. Logistic regression analysis was used to establish a model to predict the risk of recurrence or death within 5 years. Results compared with the non-low expression group, the low expression of 10 m RNA in the PCDH10 m group was compared with that in the non-low expression group. No progressive survival time (PFSs) and total survival time (total survival time) significantly prolonged P values were 0.046 and 0.033, respectively. The lymph node metastasis was less P0. 001 and TNM staging was earlier than P0. 001. Cox univariate analysis showed that M stage, PCDH10 m RNA expression level and T stage M stage and PCDH10 m RNA expression level of T stage were detected. PFS and OS were significantly correlated. The predictive efficiency of the Logistic regression model with PCDH10 as a parameter for predicting the risk of recurrence or death in patients with gastric cancer within 5 years after operation was comparable to that of the Logistic regression model with only traditional clinicopathological parameters. Conclusion low expression of 10% PCDH10 in gastric cancer patients with lower lymph node metastasis and early TNM staging may be used as an auxiliary index to predict the prognosis of gastric cancer patients. The Logistic regression model based on the level of PCDH10 expression can help to judge the prognosis of patients with unknown lymph node metastasis.
【作者单位】：复旦大学附属肿瘤医院肿瘤内科复旦大学上海医学院肿瘤学系;上海交通大学医学院附属瑞金医院肿瘤科;同济大学附属天佑医院肿瘤科;
【基金】：国家科技重大专项基金(2012ZX09303-018-002)
【分类号】：R735.2

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