STC2促进人肝癌细胞HepG2增殖和EMT相关的迁移

发布时间：2018-06-08 03:39

本文选题：肝细胞癌 + 斯钙素　；参考：《中国病理生理杂志》2017年06期

【摘要】：目的:探讨斯钙素2(STC2)对人肝癌细胞HepG2增殖、迁移以及上皮-间充质转化(EMT)进程的影响。方法:Western blot法检测不同肝癌细胞株及正常肝细胞株的STC2蛋白表达情况;集落形成实验分析STC2对HepG2细胞增殖的影响,同时进一步采用实时荧光定量PCR及Western blot法检测STC2对cyclin D1等增殖相关基因的表达变化;Transwell实验分析STC2对肝癌细胞HepG2迁移能力的影响,采用实时荧光定量PCR和Western blot法检测过表达和沉默STC2的细胞中EMT分子标志物vimentin和E-cadherin的表达情况。结果:与正常肝细胞系相比,STC2蛋白在肝癌细胞中高表达。集落形成实验结果说明STC2促进HepG2细胞的增殖,同时STC2可以显著影响cyclin D1等增殖相关基因的表达。Transwell实验结果说明STC2增强HepG2细胞的迁移能力,同时显著影响肝癌细胞的EMT过程。结论:STC2能够促进肝癌细胞系HepG2的增殖并且影响增殖相关基因的表达,进一步研究表明STC2能够影响肝癌细胞的EMT过程,促进肝癌细胞的迁移。
[Abstract]:Aim: to investigate the effects of staphylococcin 2 (STC 2) on the proliferation, migration and epithelial-mesenchymal transformation (EMT) of human hepatoma cell line HepG2. Methods the expression of STC2 protein in different hepatoma cell lines and normal hepatocytes was detected by Western blot assay, and the effect of STC2 on the proliferation of HepG2 cells was analyzed by colony forming assay. At the same time, real-time fluorescent quantitative PCR and Western blot were used to detect the expression of cyclin D1 and other proliferation-related genes. Transwell experiment was used to analyze the effect of STC2 on the migration ability of HepG2 cells. The expression of EMT markers vimentin and E-cadherin were detected by real-time fluorescent quantitative PCR and Western blot. Results: compared with the normal liver cell line, the expression of STC2 protein was higher in hepatoma cells. The results of colony formation experiment showed that STC2 could promote the proliferation of HepG2 cells, and STC2 could significantly affect the expression of proliferation-related genes such as cyclin D1. The results showed that STC2 enhanced the migration ability of HepG2 cells and significantly affected the EMT process of hepatoma cells. Conclusion: 1. STC2 can promote the proliferation of hepatoma cell line HepG2 and affect the expression of proliferation-related genes. Further studies have shown that STC2 can affect the EMT process of HCC cells and promote the migration of HCC cells.
【作者单位】：南方医科大学病理生理学教研室广东省蛋白质组学重点实验室;南方医科大学南方医院呼吸科;
【基金】：国家自然科学基金资助项目(No.81372030) 广州市科技计划项目(No.201607020016) 南方医科大学南方医院院长基金(No.2015Z005)
【分类号】：R735.7

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