氧化损伤对SCC-25细胞增殖抑制的研究

发布时间：2018-06-11 21:53

本文选题：过氧化氢 + 氧化应激　；参考：《实用口腔医学杂志》2017年06期

【摘要】：目的:观察外源性的氧化刺激作用下诱导口腔癌细胞中mtDNA损伤、修复的动态变化过程及其对细胞增殖的影响。方法:选用口腔鳞状细胞癌SCC-25细胞株和纤维原细胞株BJ作为对照实验模型,以0(对照组)、120、240、480、960μmol/L H_2O_2处理细胞,不同时间点用ss-qPCR方法测定mtDNA损伤、修复的动态过程;MTT法测定细胞增殖活性。结果:SCC-25细胞经大于和等于120μmol/L H_2O_2处理后mtDNA损伤呈剂量依赖性增加,氧处理后2~24 h 120μmol/L组mtDNA恢复至对照组水平,其他组mtDNA损伤率逐渐下降。BJ细胞经大于和等于240μmol/L H_2O_2处理后,mtDNA损伤呈剂量依赖性增加,960μmol/L组2种细胞mtDNA损伤修复均不明显。细胞增殖率呈剂量依赖性降低。BJ细胞的类似反应程度弱于SCC-25细胞。结论:氧化刺激可能是一种重要的致癌因素。
[Abstract]:Aim: to observe the dynamic process of mtDNA damage and repair in oral cancer cells induced by exogenous oxidative stimulation and its effect on cell proliferation. Methods: SCC-25 cell line and fibroblast cell line BJ of oral squamous cell carcinoma were used as control model. The cells were treated with 0 (control group) 1202404 80960 渭 mol / L H _ 2O _ 2, and mtDNA damage was detected by ss-qPCR at different time points. The dynamic process of repair was determined by MTT assay. Results the mtDNA damage was increased in a dose-dependent manner after being treated with or equal to 120 渭 mol / L H _ 2O _ 2, and the mtDNA of 120 渭 mol / L group returned to the control level at 24 h after oxygen treatment. The mtDNA damage rate of other groups decreased gradually. The mtDNA damage of BJ cells was increased in a dose-dependent manner after being treated with or equal to 240 渭 mol / L H2O2. The mtDNA damage repair was not obvious in the two groups (960 渭 mol / L). The cell proliferation rate decreased in a dose-dependent manner. The similar reaction of BJ cells was weaker than that of SCC-25 cells. Conclusion: oxidative stimulation may be an important carcinogenic factor.
【作者单位】：锦州医科大学;
【基金】：国家自然科学基金(编号:81472746) 锦州医科大学校长基金-全民口腔资助(编号:QM2014008)
【分类号】：R739.8

【相似文献】