EGFR突变的肺腺癌患者中Rb和磷酸化Rb的表达及临床特征分析

发布时间：2018-06-13 04:21

本文选题：肺肿瘤 + Rb　；参考：《中国肺癌杂志》2017年05期

【摘要】：背景与目的 Rb作为重要的抑癌基因,调控细胞周期的进程。各种原因导致的Rb功能异常均可导致细胞的持续过度增殖从而导致肿瘤的发生。Rb蛋白表达缺失或减弱及过度磷酸化是Rb功能异常的重要机制。具有突变的表皮生长因子受体(epidermal growth factor receptor,EGFR)基因是肺腺癌重要的驱动基因,在肺癌的发生发展中起着重要的作用。本研究目的在于探讨Rb在EGFR突变的肺腺癌中的存在状态。方法取23例具有EGFR突变的肺腺癌标本,用免疫组化的方法分析Rb、p Rb-780、p Rb-795表达状态及临床特征。结果在23例EGFR突变的肺腺癌患者中Rb蛋白表达缺失/减弱频率为69.6%,p Rb-780、p Rb-795过表达的频率分别为73.9%、69.6%。23例患者均存在Rb表达缺失/减弱或Rb过度磷酸化。进一步分析发现,p Rb-780过表达在晚期患者中发生更多(P=0.022);p Rb-795过表达在晚期患者中发生更多,但无统计学差异(P=0.074)。结论在EGFR突变的肺腺癌患者中,频繁发生Rb的表达缺失/减弱或过度磷酸化,Rb功能异常是EGFR突变肺腺癌患者重要的发病机制。
[Abstract]:Background & objective RB, as an important tumor suppressor gene, regulates the progress of cell cycle. The abnormal function of RB caused by various causes can lead to the sustained excessive proliferation of cells, which leads to the loss or weakening of the expression of .RB protein and excessive phosphorylation, which is an important mechanism of abnormal function of RB. Epidermal growth factor receptor (EGFR) gene with mutation is an important driving gene of lung adenocarcinoma and plays an important role in the occurrence and development of lung cancer. The aim of this study was to investigate the presence of RB in EGFR mutant lung adenocarcinoma. Methods 23 cases of lung adenocarcinoma with EGFR mutation were collected and the expression and clinical features of RbtP Rb-780 pRb-795 were analyzed by immunohistochemical method. Results in 23 cases of lung adenocarcinoma with EGFR mutation, the frequency of deletion / abatement of RB protein expression was 69.6%. The frequency of overexpression of RB protein was 73.9%. Further analysis showed that the overexpression of pRb-780 was more frequent in the late stage patients than that in the late stage patients, but there was no statistical difference between the two groups. Conclusion in patients with lung adenocarcinoma with EGFR mutation, the frequent occurrence of deletion / weakening of RB expression or abnormal function of hyperphosphorylated RB is an important pathogenesis of lung adenocarcinoma with EGFR mutation.
【作者单位】：天津医科大学总医院肺部肿瘤外科;天津市肺癌研究所天津市肺癌转移与肿瘤微环境实验室;
【基金】：国家自然科学基金(No.81172233、No.81372306) 天津自然科学基金重点项目(No.16JCZDJC34200);天津自然科学基金项目(No.13JCYBJC22600) 天津市创新平台专项市级重点实验室建设项目(No.16PTSYJC00160) 教育部博士点基金(No.20131202110004)的资助~~
【分类号】：R734.2

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