基于γδ T细胞识别的应激配体的肝癌分子标记物研究

发布时间：2018-06-14 12:25

本文选题：肝癌 + γδ　；参考：《免疫学杂志》2017年05期

【摘要】：目的本研究从γδT细胞识别的应激配体出发,探索肝癌细胞的分子标记物。方法流式细胞技术检测了3个人肝癌细胞系(HepG2、SMMC7721和Hep3B)和1个正常的人胚肝细胞系(ccc HEL-1)表面11种已知的γδT细胞识别的应激配体的表达情况。收集了9名肝癌患者和10个健康对照的外周血样品,流式细胞技术分析了患者和对照的γδT细胞及其亚型的比例,ELISA检测血浆中10种应激配体的质量浓度。结果 3种不同的肝癌细胞系应激配体的表达情况存在着差异,与正常胚肝细胞系相比,ULBP1、ULBP5、HSP60、HSP90和MSH2在3种肝癌细胞系表达都有上调;与正常对照相比,肝癌患者外周血的γδT细胞总比例显著升高,CD27~-γδT细胞升高,而外周血主要的Vδ2细胞比例显著降低;10种应激配体在血浆中的质量浓度差异很大,在血浆质量浓度较高的应激配体中,ULBP1、ULBP4和HSP90在肝癌患者血浆中较正常人升高,有统计学意义。结论γδT细胞识别的应激配体ULBP1和HSP90在肝癌细胞系表面上调表达,并且在肝癌患者血浆中的质量浓度显著升高,是肝癌比较可靠的分子标记物。
[Abstract]:Objective to explore the molecular markers of hepatoma cells from the stress ligand recognized by 纬未 T cells. Methods flow cytometry was used to detect the expression of 11 known stress ligands recognized by 纬未 T cells on the surface of three human hepatoma cell lines (HepG2SMC 7721 and Hep3B) and one normal human embryonic liver cell line (HEC HEL-1). Peripheral blood samples from 9 patients with liver cancer and 10 healthy controls were collected. The proportion of 纬未 T cells and their subtypes in patients and controls were analyzed by flow cytometry. The mass concentrations of 10 stress ligands in plasma were detected by Elisa. Results the expression of stress ligands in three different hepatoma cell lines was different. Compared with normal embryonic liver cell line, the expression of HSP90 and MSH2 in HSP60HSP90 and MSH2 were up-regulated in all three hepatoma cell lines, and compared with those in normal control, the expression of HSP90 and MSH2 in HSP60HSP90 and MSH2 were up-regulated in three hepatoma cell lines. The percentage of 纬未 T cells in peripheral blood of patients with liver cancer was significantly increased, while the percentage of major V 未 2 cells in peripheral blood significantly decreased the mass concentration of 10 stress ligands in plasma. In the stress ligands with higher plasma mass concentration, ULBP1UBP4 and HSP90 were significantly higher in HCC patients than in normal controls. Conclusion the expression of ULBP1 and HSP90, the stress ligand recognized by 纬未 T cells, is up-regulated on the surface of HCC cell lines, and the mass concentration in plasma of HCC patients is significantly increased, which is a reliable molecular marker for HCC.
【作者单位】：中国医学科学院基础医学研究所国家分子生物学重点实验室北京协和医科大学基础学院;
【基金】：医科院基本科研业务项目(2016ZX310180-5) 国家自然科学基金(31500725,81572804,81673010) 十二五国家科技重大专项(2012ZX10002015008)
【分类号】：R735.7

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