miR-7-5p靶向调控EGFR对非小细胞肺癌细胞增殖侵袭的影响

发布时间：2018-06-17 00:50

本文选题：非小细胞肺癌 + 微小RNA--p　；参考：《山东医药》2017年45期

【摘要】：目的探讨miR-7-5p是否通过调控表皮生长因子受体(EGFR)的表达而影响人非小细胞肺癌细胞体外增殖和侵袭能力。方法通过双荧光素酶试验证明EGFR为miR-7-5p的下游靶基因,用miR-7-5p(miR-7-5p组)或miR-N.C.(miR-N.C.组)转染NCI-H1975细胞,并设立空白对照组,采用实时荧光定量PCR法检测EGFR mRNA,采用Western blotting法检测EGFR蛋白的表达。然后通过比色法、流式细胞术和Transwell小室法检测NCIH1975细胞体外增殖活力和侵袭能力。结果 miR-7-5p可通过靶向3'UTR发挥调节作用,降低荧光素酶的表达活性。miR-7-5p组EGFR mRNA及蛋白表达量低于miR-N.C.组(P均0.05)。转染后72 h,miR-7-5p组细胞增殖活力比miR-N.C.组降低(P0.05)。与miR-N.C.组相比,miR-7-5p组NCI-H1975细胞S期所占比例下调(P0.05),而G0/G1期细胞所占比例增加(P0.05)。与miR-N.C组相比,miR-7-5p组细胞侵袭变化率下调(P0.05)。结论 miR-7-5p通过靶向抑制EGFR的表达而抑制人非小细胞肺癌细胞的增殖和侵袭能力。
[Abstract]:Objective to investigate whether miR-7-5p affects the proliferation and invasion of human non-small cell lung cancer cells in vitro by regulating the expression of epidermal growth factor receptor (EGFR). Methods EGFR was proved to be the downstream target gene of miR-7-5p by double luciferase assay. NCI-H1975 cells were transfected and blank control group was set up. EGFR mRNAs were detected by real-time fluorescence quantitative PCR and EGFR protein expression was detected by Western blotting assay. Then the proliferative activity and invasiveness of NCIH1975 cells in vitro were detected by colorimetry, flow cytometry and Transwell chamber assay. Results miR-7-5p could inhibit the expression of luciferase by targeting 3UTR. The expression of EGFR mRNA and protein in miR-7-5p group was lower than that in miR-N.Cgroup. The P of the group was 0.05. The proliferative activity of miR-7-5p group was higher than that of miR-N.C. In group A, P 0.05 was decreased. And miR-N.C. The percentage of S phase of NCI-H1975 cells was down-regulated in the group of miR-7-5p, while the proportion of cells in the G _ 0 / G _ 1 phase increased. Compared with miR-N.C group, the cell invasion rate of miR-7-5p group was down-regulated (P 0.05). Conclusion miR-7-5p inhibits the proliferation and invasion of human non-small cell lung cancer cells by targeting EGFR expression.
【作者单位】：承德医学院附属医院;
【分类号】：R734.2

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