苯丁酸钠对多烯紫杉醇耐药前列腺癌细胞株的增值抑制和凋亡诱导作用
发布时间：2018-06-17 03:51
本文选题：前列腺癌 + 化疗耐药 ； 参考：《南方医科大学学报》2017年01期
【摘要】：目的研究苯丁酸钠对前列腺癌多烯紫杉醇耐药细胞株的增值抑制与凋亡诱导作用,并探讨其作用机制。方法构建多烯紫杉醇耐药前列腺癌PC3细胞株(PC3/DTX),采用0、1、2、4 mmol/L的苯丁酸钠处理PC3/DTX,MTT检测细胞增殖、细胞活力以及抑制率,计算不同浓度苯丁酸钠处理后各组多烯紫杉醇对PC3/DTX细胞的半致死浓度(IC50);流式细胞仪检测不同处理组细胞凋亡水平;Western blot检测p21、Cyclin D1与Survivin的表达水平。结果 0、1、2、4 mmol/L的苯丁酸钠处理PC3/DTX细胞48 h后,细胞存活率分别是(99.85±2.69)%,(84.68±3.87)%,(68.65±4.54)%和(43.54±5.69)%(P0.05);抑制率分别是(10.69±3.65)%,(5.78±4.58)2%,(54.68±3.98)%和(69.84±6.54)%(P0.05);多烯紫杉醇对各处理组细胞的IC50分别为135.98±2.69、109.65±3.87、87.65±3.84、64.62±2.98 nmol/L(P0.05);细胞的凋亡率分别为(7.20±0.80)%,(10.20±0.90)%,(19.80±2.10)%和(27.40±2.50)%(P0.05);Western blot显示苯丁酸钠促进p21蛋白的表达,抑制Cyclin D1与Survivin蛋白的表达。结论苯丁酸钠可以增加前列腺癌耐药细胞株对多烯紫杉醇的药敏性,并影响前列腺癌耐药细胞株p21、Cyclin D1与Survivin蛋白的表达量。
[Abstract]:Aim to study the effect of sodium phenylbutyrate on proliferation inhibition and apoptosis induction of docetaxel resistant prostate cancer cell line. Methods PC3 / DTXT cell line was constructed and treated with sodium phenylbutyrate of 4 mmol / L to detect cell proliferation, cell viability and inhibition rate. The half-lethal concentration of docetaxel on PC3 / DTX cells was calculated after different concentrations of sodium phenylbutyrate, the apoptosis level of PC3 / DTX cells was detected by flow cytometry and the expression levels of p21 Cyclin D1 and survivin were detected by flow cytometry. Results PC3 / DTX cells were treated with 4 mmol / L sodium phenylbutyrate for 48 h. 缁嗚優瀛樻椿鐜囧垎鍒槸(99.85卤2.69)%,(84.68卤3.87)%,(68.65卤4.54)%鍜，
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