胃癌患者T淋巴细胞表面PD-1分子的表达水平及临床意义

发布时间：2018-06-18 06:01

本文选题：胃癌 + PD-1　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:运用流式细胞术检测胃癌患者外周血T细胞亚群及CD4+T细胞、CD8+T细胞表面程序性死亡受体-1(programmed death1,PD-1)的表达情况及其与胃癌患者临床病理特征的关系,探究其临床意义。方法:收集山西医科大学附属肿瘤医院于2015年6月至2016年6月入院且初诊胃癌患者169例、健康对照组68例,采用流式细胞术检测其外周血T淋巴细胞亚群和CD4+T、CD8+T淋巴细胞表面PD-1的表达水平,并分析手术患者PD-1表达与其TNM分期、浸润层面、淋巴结转移、脉管癌栓、神经受侵及肿瘤体积的关系。结果:1.胃癌患者外周血T淋巴细胞CD3、CD4表达水平显著低于健康体检组(P0.001),CD8的表达则高于健康体检组(P0.001);2.胃癌患者外周血CD4+T、CD8+T淋巴细胞表面PD-1的表达显著高于健康对照组(P0.001);3.CD4+T细胞表面PD-1表达与TNM分期(P0.001)、淋巴结转移(P=0.007)、肿瘤体积(P=0.039)表达相关,与浸润深度(P=0.402)、脉管癌栓(P=0.609)和神经受侵(P=0.253)不相关;CD8+T细胞表面PD-1表达与TNM分期(P0.001)、淋巴结转移(P=0.004)表达相关,与浸润层面(P=0.94)、脉管癌栓(P=0.712)、神经受侵(P=0.671)及肿瘤体积(P=0.165)表达不相关。结论:胃癌患者外周血CD3+T淋巴细胞、CD4+T淋巴细胞表达降低,CD8+T淋巴细胞升高,并且CD4+T PD-1+、CD8+T PD-1+细胞表达显著增高,并与胃癌患者临床病理特征有相关性,PD-1的表达水平与胃癌的免疫逃逸过程有关,并且在胃癌的发生发展中起至关重要的作用。
[Abstract]:Objective: to detect the expression of peripheral blood T cell subsets and CD4 T cell / CD8 T cell surface programmed death receptor (PCD 1) in patients with gastric cancer by flow cytometry (FCM) and its relationship with the clinicopathological features of gastric cancer patients, and to explore its clinical significance. Methods: 169 patients with gastric cancer and 68 healthy controls were collected from June 2015 to June 2016 in affiliated Cancer Hospital of Shanxi Medical University. The expression of PD-1 on peripheral blood T lymphocyte subsets and CD4 / CD8 T lymphocytes was detected by flow cytometry, and the expression of PD-1 was analyzed with TNM staging, invasive layer, lymph node metastasis, vascular tumor thrombus. The relationship between nerve invasion and tumor volume. The result is 1: 1. The expression of CD3T CD4 in peripheral blood of patients with gastric cancer was significantly lower than that in healthy controls (P 0.001) and CD8 was higher than that in healthy controls (P 0.001). The expression of PD-1 on CD4 / CD8 T lymphocytes in peripheral blood of patients with gastric cancer was significantly higher than that on normal controls. 3. The expression of PD-1 on CD4 T cells was correlated with the expression of P0.001, P0. 007 and P0. 039 in TNM staging, lymph node metastasis and tumor volume. There was no correlation between the expression of PD-1 on the surface of CD8 T cells and the expression of P0.001 in TNM staging, P0.001 in lymph node metastasis (P0.004), P0. 94 in invasive layer, P0. 712 in vascular tumor thrombus, P0. 671 in nerve invasion and P0. 165) in tumor volume. The expression of PD-1 on the surface of CD8 T cells was not correlated with the expression of P0. 001, P0. 004), P0. 712, P0. 671) and P0. 165). Conclusion: the expression of CD 3 T lymphocytes in peripheral blood of patients with gastric cancer is lower than that of CD 8 T lymphocytes, and the expression of CD 4 T PD-1 T CD 8 T PD-1 cells is significantly higher than that of CD 4 T PD-1 T cells. The expression level of PD-1 is related to the immune escape process of gastric cancer and plays an important role in the occurrence and development of gastric cancer.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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