Runx2通过促进MMP9的表达增强肝细胞肝癌迁移侵袭能力

发布时间：2018-06-19 18:32

  本文选题：肝细胞肝癌 + Runx　； 参考：《中国肿瘤临床》2017年09期

【摘要】：目的:研究Runx2对肝癌细胞中MMP9的表达以及其对肝癌细胞侵袭迁移能力的影响。方法:通过免疫组织化学染色法对2005年12月至2015年12月天津医科大学肿瘤医院和天津医科大学总医院189例肝细胞肝癌病例标本中Runx2和MMP9的表达进行分析;将Runx2过表达质粒和干扰质粒分别转染至肝癌细胞系HepG2和SMMC7721细胞中。使用Western blot法检测转染后HepG2和SMMC7721细胞中Runx2和MMP9的表达情况;划痕、侵袭实验检测Runx2对肝癌细胞迁移和侵袭能力的影响。结果:免疫组织化学结果显示Runx2的表达和MMP9的表达相关。在HepG2细胞中上调Runx2的表达,促进了MMP9的表达,增强了HepG2细胞迁移侵袭能力;在SMMC7721细胞中下调Runx2的表达,降低了MMP9的表达,抑制了SMMC7721细胞的迁移侵袭能力。结论:Runx2的表达可能参与促进肝细胞肝癌细胞MMP9的表达,进而促进肝细胞肝癌的迁移侵袭能力。
[Abstract]:Aim: to study the effect of Runx2 on the expression of MMP9 and the ability of invasion and migration of hepatoma cells. Methods: from December 2005 to December 2015, the expression of Runx2 and MMP9 in 189 cases of hepatocellular carcinoma (HCC) in Tianjin Medical University Oncology Hospital and Tianjin Medical University General Hospital were analyzed by immunohistochemical staining. Runx2 overexpression plasmid and interference plasmid were transfected into hepatoma cell lines HepG2 and SMMC7721, respectively. The expression of Runx2 and MMP9 in transfected HepG2 and SMMC7721 cells was detected by Western blot assay, and the effect of Runx2 on the migration and invasion of hepatoma cells was detected by scratch and invasion assay. Results: immunohistochemical results showed that the expression of Runx 2 was correlated with the expression of MMP9. The expression of Runx2 was up-regulated in HepG2 cells, which promoted the expression of MMP9 and enhanced the ability of migration and invasion of HepG2 cells, and down-regulated the expression of Runx2 in SMMC7721 cells, reduced the expression of MMP9 and inhibited the migration and invasion of SMMC7721 cells. Conclusion the expression of 1: Runx2 may play an important role in promoting the expression of MMP9 in hepatocellular carcinoma cells, thus promoting the ability of migration and invasion of hepatocellular carcinoma.
【作者单位】： 天津医科大学病理教研室;天津医科大学肿瘤医院病理科;天津医科大学总医院病理科;
【基金】：国家自然基金面上项目(编号:81572872) 国家自然科学基金重点项目(编号:81230050)资助~~
【分类号】：R735.7

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