c-FLIP_L在白血病中的表达及临床意义

发布时间：2018-06-20 02:27

本文选题：c-FLIP_L + 白血病　；参考：《中国医科大学学报》2017年02期

【摘要】：目的探讨c-FLIPL在不同类型白血病中的表达水平及其临床意义。方法应用实时PCR检测103例不同类型白血病患者骨髓单个核细胞c-FLIP_L mRNA表达,包括急性淋巴细胞白血病(ALL)54例(其中初诊37例、复发5例、完全缓解12例)、急性髓细胞白血病(AML)38例(其中初诊24例、复发6例、完全缓解8例)、初诊急性未分化白血病(AUL)2例、初诊慢性粒细胞白血病(CML)6例、初诊慢性粒单核细胞白血病(CMML)3例。应用流式细胞术对所选病例进行免疫表型检测。结果在初诊和复发的白血病中c-FLIP_L mRNA呈异常高表达趋势,二者间差异无统计学意义(P0.05)。初诊AUL及初诊CML的c-FLIP_L mRNA表达高于其他初诊组,但差异无统计学意义(P0.05)。初诊ALL、AML、AUL、CML、CMML与对照组、完全缓解组比较,c-FLIP_L mRNA的差异均有统计学意义(P0.05)。c-FLIP_L mRNA表达与患者危险度分级、初诊白细胞数、血乳酸脱氢酶(LDH)、血羟丁酸脱氢酶(HBDH)、CD45、融合基因TEL-AML1相关,与患者年龄、性别、血纤维蛋白原及染色体异常无关。结论白血病患者c-FLIP_L mRNA表达异常增高,并与危险度分级、初诊白细胞数、血LDH、血HBDH及预后密切相关。c-FLIPPCR
[Abstract]:Objective to investigate the expression of c-FLIPL in different types of leukemia and its clinical significance. Methods the expression of c-FLIPL mRNA in bone marrow mononuclear cells of 103 patients with different types of leukemia was detected by real-time PCR, including 54 cases of acute lymphoblastic leukemia (ALL), including 37 cases of initial diagnosis and 5 cases of recurrence. There were 12 cases of complete remission, 38 cases of acute myeloid leukemia (including 24 cases of initial diagnosis, 6 cases of recurrence, 8 cases of complete remission, 2 cases of initial diagnosis of acute undifferentiated leukemia and 6 cases of chronic myelogenous leukemia). Three cases of chronic myelogenous monocytic leukemia (CMMLN) were first diagnosed. Immunophenotypes of selected cases were detected by flow cytometry. Results the expression of c-FLIPL mRNA in the newly diagnosed and relapsed leukemia showed an abnormal tendency, and there was no significant difference between the two groups (P 0.05). The expression of c-FLIPL mRNA in newly diagnosed AUL and newly diagnosed CML was higher than that in other groups, but the difference was not statistically significant (P 0.05). The expression of c-FLIPL mRNA in the complete remission group was significantly different from that in the control group. The expression of c-FLIPL mRNA was correlated with the risk grade of patients, the number of leukocytes at first visit, the number of blood lactate dehydrogenase (LDH), the HBDHHL-CD45, the fusion gene TEL-AML1, and the age of the patients. Sex, fibrinogen and chromosomal abnormalities were not found. Conclusion the expression of c-FLIPL mRNA in leukemic patients is abnormally high, and it is closely related to the risk grade, the number of newly diagnosed leukocytes, the blood LDH, the HBDH and the prognosis. C-FLIP PCR is closely related to the expression of c-FLIPL mRNA.
【作者单位】：中国医科大学附属盛京医院小儿血液内科;中国医科大学附属盛京医院第二血液内科;中国医科大学附属盛京医院血液研究室;
【基金】：沈阳市科技计划(F13-316-1-08)
【分类号】：R733.7

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