RSK4在非小细胞肺癌中的表达及临床病理特征的关系

发布时间：2018-06-21 16:32

本文选题：非小细胞肺癌 + RSK4　；参考：《桂林医学院》2017年硕士论文

【摘要】：目的:肺癌是目前全世界癌症相关死亡的首要原因。非小细胞肺癌(non-small cell lung cancer,NSCLC)是肺癌亚型中最常见的类型。核糖体S6蛋白激酶4(ribosomal S6 kinase 4,RSK4)作为一个蛋白激酶调节细胞生长、迁移、增殖和存活。其在肺癌中的表达及意义尚不明确。本研究通过免疫组织化学和RT-PCR检测非小细胞肺癌组织中RSK4蛋白和mRNA,探讨其在非小细胞肺癌与癌旁非瘤组织中的表达差异、其表达与临床病理特征的关系。阐明RSK4在非小细胞肺癌组织中的表达及意义。方法:选取40例2015年01月到2015年12月本院就诊的NSCLC患者纳入研究。所有的纳入研究的患者均经病理科确诊为NSCLC,并在实验前由3位中级以上病理科医师复诊无疑义者。RSK4 mRNA和蛋白分别用RT-PCR和免疫组织化学法进行检测。所有用于RT-PCR的肺癌组织标本和癌旁非瘤组织标本均在手术中获取;对应的石蜡组织标本从病理科获得。并将RSK4基因的表达水平与患者的临床病理特征进行比较。结果:与癌旁非瘤肺组织比较,RSK4 mRNA及蛋白在NSCLC组织中的表达下调(P0.01)。同时,RSK4蛋白在NSCLC组织中的含量也明显低于肺大疱患者的肺组织(P0.01)。RSK4表达下调与NSCLC的肿瘤大小、TNM分期相关;而与年龄、性别、CEA水平、腺癌或鳞癌的组织学类型无关。结论:RSK4在NSCLC中表达下调,与肿瘤大小、临床病理分期有关,提示RSK4在NSCLC中可能发挥抑癌基因的功能。
[Abstract]:Objective: lung cancer is the leading cause of cancer-related deaths worldwide. Non-small cell lung cancer (NSCLC) is the most common subtype of lung cancer. Ribosomal S6 protein kinase (4(ribosomal S6 kinase 4 RSK4) acts as a protein kinase to regulate cell growth, migration, proliferation and survival. Its expression and significance in lung cancer is unclear. In this study, the expression of RSK4 protein and mRNAs in non-small cell lung cancer (NSCLC) tissues were detected by immunohistochemistry and RT-PCR, and the expression of RSK4 protein and mRNAs in non-small cell lung cancer (NSCLC) and adjacent non-tumor tissues (NSCLC) was studied. To elucidate the expression and significance of RSK4 in non-small cell lung cancer (NSCLC). Methods: 40 NSCLC patients from January 2015 to December 2015 were included in the study. All the patients included in the study were diagnosed as NSCLC by pathology, and the mRNA and protein of RSK4 were detected by RT-PCR and immunohistochemistry respectively. All lung cancer tissue specimens and adjacent non-tumor specimens were obtained during operation, and the corresponding paraffin tissues were obtained from pathologists. The expression level of RSK4 gene was compared with the clinicopathological features of the patients. Results: the expression of RSK4 mRNA and protein was down-regulated in NSCLC tissues compared with non-cancerous lung tissues. At the same time, the expression of RSK4 protein in NSCLC was significantly lower than that in patients with bullous lung. The down-regulated expression of RSK4 was correlated with the tumor size and TNM stage of NSCLC, but not with age, sex and CEA level, histological type of adenocarcinoma or squamous cell carcinoma. Conclusion the down-regulation of the expression of RSK4 in NSCLC is related to tumor size and clinicopathologic stage, suggesting that RSK4 may play a role as a tumor suppressor gene in NSCLC.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

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