去甲肾上腺素对人肝癌细胞HepG2和人正常肝细胞L-02增殖、凋亡的影响

发布时间：2018-06-24 13:17

本文选题：去甲肾上腺素 + 人肝癌细胞HepG　；参考：《山东医药》2017年47期

【摘要】：目的观察去甲肾上腺素(NE)对人肝癌细胞HepG2和人正常肝细胞L-02增殖、凋亡的影响。方法体外培养HepG2细胞和L-02细胞,分别加入不同浓度NE处理,继续培养,采用MTT法观察细胞增殖情况,HE染色和Annexin V-FITC/PI染色法观察细胞形态学变化,流式细胞术观察细胞凋亡情况。结果经0.1、1、10、50μmol/L NE处理24、48 h,HepG2细胞OD值显著下降(P均0.05),L-02细胞OD值无明显变化。10μmol/L NE作用于HepG2细胞可见典型的凋亡形态学改变,胞质收缩、深染,核染色质浓缩;L-02细胞在NE作用前后形态学均无明显改变。HepG2细胞加入10μmol/L NE后部分细胞呈绿色(为早期凋亡表现),加入50μmol/L NE后部分细胞膜呈绿色,细胞核呈红色(为晚期凋亡表现);L-02细胞均未显示有荧光。0.1、1、10μmol/L NE作用于HepG2细胞24 h后,其凋亡率均显著高于对照组(0μmol/L NE),且呈剂量依赖关系(P均0.05);而上述浓度NE作用于L-02细胞24 h后,其凋亡率较对照组先增高后降低(P均0.05)。结论 NE可以抑制HepG2细胞增殖并诱导其凋亡,而对L-02细胞增殖和凋亡没有影响,提示其在特定类型肝癌临床治疗中具有潜在价值。
[Abstract]:Objective to observe the effects of norepinephrine (NE) on proliferation and apoptosis of human hepatoma cell line HepG2 and normal hepatocytes L-02. Methods HepG2 cells and L-02 cells were cultured in vitro and treated with different concentrations of NE respectively. Cell proliferation was observed by HE staining and Annexin V-FITC- Pi staining, and apoptosis was observed by flow cytometry. Results after treated with 0.1 渭 mol / L NE for 48 h, the OD value of HepG2 cells decreased significantly (P 0.05). The OD value of L-02 cells did not change significantly. 10 渭 mol / L NE could induce typical apoptotic morphological changes, cytosolic contraction and deep staining in HepG2 cells. There was no obvious change in morphology before and after NE treatment. After adding 10 渭 mol / L NE to HepG2 cells, some of the cells were green (early apoptosis), and some of the cells were green after adding 50 渭 mol / L NE. The apoptosis rate of L-02 cells was significantly higher than that of the control group (0 渭 mol / L NE) (P 0.05) after 24 h treatment with 10 渭 mol / L NE, and the concentration of NE was significantly higher than that of the control group (P 0.05), but the concentration of NE was significantly higher than that of the control group (P 0.05), and the cell apoptosis rate was significantly higher than that of the control (0 渭 mol / L NE) (P 0.05), but the concentration of NE was significantly higher than that of the control group (P 0.05). Compared with the control group, the apoptotic rate increased first and then decreased (P 0.05). Conclusion NE can inhibit the proliferation and induce apoptosis of HepG2 cells, but has no effect on the proliferation and apoptosis of L-02 cells.
【作者单位】：苏州大学附属第三医院;常州市第一人民医院;
【基金】：江苏省常州市国际科技合作项目(CZ20160013) 常州市卫生局指导性科技项目(WZ200901) 江苏省青年医学人才(QNRC2016282)
【分类号】：R735.7

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