MIG7调节肝细胞癌中血管生成拟态的形成及其对肝癌转移潜能的影响

发布时间：2018-06-24 21:11

本文选题：血管生成拟态 + 迁移诱导基因　；参考：《四川大学学报(医学版)》2017年03期

【摘要】：目的探讨肝细胞癌(HCC)中迁移诱导基因7(MIG7)的表达与血管生成拟态(VM)形成的相关性,以及MIG7的表达与不同肝癌细胞株VM形成能力及转移潜能的关系。方法应用免疫组织化学方法分析配对的40例HCC原发灶及其转移灶标本中MIG7蛋白的表达和VM形成情况;应用三维培养技术培养不同转移潜能的人肝癌细胞株(高转移潜能肝癌细胞MHCC-97H、低转移潜能肝癌细胞MHCC-97L和非转移肝癌细胞Huh-7)及人正常肝细胞L-02,分别检测VM形成情况;应用RT-PCR技术检测不同转移潜能肝癌细胞中MIG7mRNA的水平;应用Western blot技术检测不同转移潜能肝癌细胞株中MIG7蛋白表达水平。结果 (1)40例配对的HCC原发灶和相应转移灶中,MIG7蛋白的表达与VM形成呈正相关(rs=0.595,P0.01)。对照组中正常肝脏组织MIG7蛋白无表达,且未见VM形成。(2)体外三维培养提示,高转移潜能肝癌细胞株MHCC-97H的MIG7mRNA和蛋白表达及VM的形成能力均大于低转移潜能肝癌细胞株MHCC-97L及非转移肝癌细胞株Huh-7。人正常肝细胞L-02未见VM形成。结论 MIG7高表达的肝癌组织中VM形成能力强,MIG7通过调节VM的形成影响HCC的侵袭转移,MIG7是抑制HCC侵袭转移的潜在靶点。
[Abstract]:Objective to investigate the relationship between the expression of migration inducible gene 7 (MIG7) and angiogenesis mimicry (VM) in hepatocellular carcinoma (HCC), and the relationship between MIG7 expression and VM formation and metastasis potential of different HCC cell lines. Methods Immunohistochemical method was used to analyze the expression of MIG7 protein and the formation of VM in 40 matched specimens of HCC. Human hepatocellular carcinoma cell lines with different metastatic potential (MHCC-97H, MHCC-97L and Huh-7) and normal hepatocytes (L-02) with different metastatic potential were cultured by three-dimensional culture technique. The expression of MIG7 mRNA in hepatocellular carcinoma cells with different metastatic potentials was detected by RT-PCR, and the expression of MIG7 protein in hepatocellular carcinoma cell lines with different metastatic potentials was detected by Western blot. Results (1) there was a positive correlation between the expression of MIG7 protein and VM formation in 40 matched HCC primary and corresponding metastatic lesions (r sr 0.595U P0.01). There was no expression of miG7 protein and no VM formation in normal liver tissue in control group. (2) Three-dimensional culture in vitro suggested that MIG7 protein was not expressed in normal liver tissue. The expression of MIG7 mRNA and protein and the ability of VM formation in MHCC-97H cell line were higher than those in MHCC-97L cell line and Huh-7 cell line. No VM was found in normal hepatocytes L-02. Conclusion the ability of VM formation in HCC tissues with high MIG7 expression is strong. MIG7 may be a potential target for inhibiting the invasion and metastasis of HCC by regulating the formation of VM.
【作者单位】：武警后勤学院附属医院肿瘤外科锦州医科大学武警后勤学院附属医院培养基地;西安交通大学第一附属医院肝胆外科;武警后勤学院附属医院救援医学研究所;武警后勤学院附属医院病理科;
【基金】：国家自然科学基金面上项目(No.81272547)资助
【分类号】：R735.7

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