SOXⅡ核蛋白表达与套细胞淋巴瘤预后的关系

发布时间：2018-06-25 05:02

  本文选题：SOX11核表达 + MCL　； 参考：《南昌大学》2015年硕士论文

【摘要】：目的:研究探索SOXll核蛋白表达与套细胞淋巴瘤预后的关系。方法:1、总共收集到我院、江西省人民医院2002年1月至2011年12月收治的102例MCL患者资料。2、最终符合入组标准者48例;男性30例,女性18例。3、采集48例MCL患者病理组织切片,经烤片、脱蜡、水化等处理后,滴加入S0X11单克隆抗体(American Santa Cruz)。4、显微镜下观察MCL肿瘤细胞S0X11核表达,细胞核可见到棕黄色细颗粒者是阳性染色(部分切片合并细胞质染色阳性),肿瘤细胞未出现阳性染色则是阴性。5、每张阳性切片随机选取5个视野(400倍),计算出每个视野阳性细胞百分比,5个百分比的平均值为该病例S0X11核表达率。6、结合相关文献,参考十三点评分法,将有阳性细胞的切片判定为S0X11核表达阳性,没有阳性细胞的切片判定为S0X11核表达阴性。7、参考十三点评分法,将病例分为阴性组(S0X11核表达阴性),弱阳性组(S0X11核表达阳性率50%),阳性组(50%≤S0X11核表达阳性率80%),强阳性组(S0X11核表达阳性率≥80%)。8、对48例MCL病例进行随访,明确每例病例的总生存期(OS)。9、应用Cox回归分析,对阴性、弱阳性、阳性、强阳性四组病例生存时间进行分析,先后使用了分类变量、连续变量两种方式分析处理。结果:1、弱阳性组、阳性组、强阳性组与阴性组比较生存时间没有显著性差异(P0.05)。2、随着SOXII核表达阳性率增加,死亡危险显著性增加(P0.05)。结论:MCL患者SOX11核蛋白表达水平与预后呈负相关,SOX11核蛋白表达率越高预后越差。
[Abstract]:Objective: to investigate the relationship between SOXll nuclear protein expression and prognosis of mantle cell lymphoma. Methods A total of 102 cases of MCL patients admitted to Jiangxi Provincial people's Hospital from January 2002 to December 2011 were collected. Data of 102 patients with MCL were collected from January 2002 to December 2011. Finally, 48 patients with MCL were included in the study, including 30 males, 18 females, and 48 patients with MCL. After treated with roasted slice, dewaxing and hydration, S0X11 monoclonal antibody (American Santa Cruz). 4 was added. The nuclear expression of S0X11 was observed under microscope. The cells with fine brown granules were positive (some sections were positive with cytoplasm staining), but the tumor cells were negative. 5 fields (400 times) were randomly selected for each positive section. The average of 5 percent of positive cells was S0X11 nuclear expression rate. Referring to the 13:00 scoring method, the sections with positive cells were determined to be positive for S0X11 nuclear expression, while sections without positive cells were determined to be negative for S0X11 nuclear expression. The patients were divided into negative group (negative expression of S0X11 nucleus), weak positive group (50% of S0X11 nuclear expression), positive group (50% 鈮，

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