miR-21通过PDCD4调控肝癌细胞的生长和侵袭

发布时间：2018-06-27 13:09

本文选题：肝肿瘤 + miR-　；参考：《临床与实验病理学杂志》2017年04期

【摘要】：目的探讨miR-21在原发性肝细胞癌(hepatocellular carcinoma,HCC)组织和细胞中的表达及其可能调控的靶基因。方法检测miR-21在HCC组织和细胞系中的表达,使用miR-21抑制剂后,观察HCC细胞活力和侵袭能力的变化;运用生物信息学分析预测miR-21可能的靶基因。应用miR-21抑制剂后,双荧光素酶报告基因系统检测其对靶基因活性的影响。结果HCC组织中miR-21表达水平显著高于癌旁组织(P0.05);HCC细胞中miR-21的表达水平显著高于肝细胞(P0.01)。抑制miR-21后,HCC细胞的活力和侵袭能力降低(P0.01)。抑癌基因程序性死亡因子4(programmed cell death 4,PDCD4)在HCC组织中的表达显著低于癌旁组织(P0.01),在肝癌细胞中的表达水平显著低于肝细胞(P0.01)。干扰PDCD4后,肝癌细胞的活力和侵袭能力提高(P0.05)。双荧光素酶报告基因检测显示,使用miR-21抑制剂后,PDCD4表达上调(P0.01),肝癌细胞的侵袭和增殖能力降低(P0.01)。干扰PDCD4后,肝癌细胞的侵袭和增殖能力升高(P0.001)。结论miR-21可通过PDCD4调控肝癌细胞的生长和侵袭。
[Abstract]:Objective to investigate the expression of miR-21 in tissues and cells of primary hepatocellular carcinoma (hepatocellular) and its possible regulatory target genes. Methods the expression of miR-21 was detected in HCC tissues and cell lines. After miR-21 inhibitor was used, the activity and invasiveness of HCC cells were observed, and the possible target genes of miR-21 were predicted by bioinformatics analysis. After using miR-21 inhibitor, double luciferase reporter gene system was used to detect the effect of miR-21 inhibitor on target gene activity. Results the expression level of miR-21 in HCC was significantly higher than that in HCC (P0.05) and the expression of miR-21 in HCC was significantly higher than that in hepatocytes (P0.01). Inhibition of the viability and invasion of HCC cells after miR-21 (P0.01). The expression of tumor suppressor gene PDCD4 in HCC tissues was significantly lower than that in adjacent HCC tissues (P0.01), and the expression level in HCC cells was significantly lower than that in HCC cells (P0.01). After interfering with PDCD4, the viability and invasive ability of hepatoma cells were increased (P0.05). The detection of double luciferase reporter gene showed that the expression of PDCD4 was up-regulated (P0.01) and the invasion and proliferation of hepatoma cells was decreased (P0.01) after miR-21 inhibitor was used. After interfering with PDCD4, the invasion and proliferation of hepatoma cells increased (P0.001). Conclusion miR-21 can regulate the growth and invasion of hepatoma cells through PDCD4.
【作者单位】：江苏省南通市第一人民医院肿瘤科;
【基金】：南通市卫计委青年基金(WQ2015018)
【分类号】：R735.7

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