锌对于原发性肝细胞癌BEL-7404细胞生物学行为的影响

发布时间：2018-07-01 14:07

本文选题：肝细胞癌 + BEL-细胞　；参考：《中国病理生理杂志》2017年12期

【摘要】：目的:观察外源锌对原发性肝细胞癌(HCC)BEL-7404细胞生物学行为的影响。方法:应用TSQ锌离子荧光探针、MTT法、DNA倍体法、吖啶橙/溴乙啶双荧光染色法和Transwell小室法分别检测不同浓度硫酸锌刺激下BEL-7404细胞内锌离子含量、细胞活力、细胞周期、细胞凋亡及细胞迁移和侵袭能力的变化。应用real-time PCR和Western blot法分别检测不同浓度锌离子对BEL-7404细胞白蛋白的mRNA和蛋白表达的影响。结果:随着培养环境中锌离子浓度的升高,BEL-7404细胞内的锌离子含量增加,细胞的存活率和细胞迁移与侵袭能力降低,凋亡率升高(P0.05);G0/G1期细胞比例降低,G2/M期细胞比例升高(P0.05);细胞白蛋白的mRNA和蛋白表达量增加(P0.05)。结论:外源性给予锌离子可抑制HCC细胞的活力、迁移与侵袭能力,诱导细胞凋亡,阻滞细胞周期在G2/M期,并可能降低细胞的恶性表型。
[Abstract]:Aim: to observe the effect of exogenous zinc on the biological behavior of BEL-7404 cells in primary hepatocellular carcinoma (HCC). Methods: MTT assay, acridine orange / bromoethidime double fluorescence staining and Transwell chamber assay were used to detect the zinc content, cell viability and cell cycle of BEL-7404 cells stimulated by zinc sulfate at different concentrations. Apoptosis and the changes of cell migration and invasion ability. The effects of zinc ions at different concentrations on the expression of albumin in BEL-7404 cells were detected by real-time PCR and Western blot. Results: with the increase of zinc concentration in culture environment, the content of zinc ions in BEL-7404 cells increased, and the cell survival rate and cell migration and invasion ability decreased. Apoptosis rate increased (P0.05) the proportion of cells in G _ 0 / G _ 1 phase decreased and the proportion of G _ 2 / M phase cells increased (P0.05), and the mRNA and protein expression of albumin increased (P0.05). Conclusion: exogenous zinc ions can inhibit the viability, migration and invasion of HCC cells, induce apoptosis, block cell cycle in G _ 2 / M phase, and may decrease the malignant phenotype of HCC cells.
【作者单位】：福建医科大学省立临床医学院;福建省立医院检验科;福建省立医院肝胆外科;
【基金】：福建省科技计划重点项目(No.2014Y0009) 福建省卫生系统中青年骨干人才培养项目(No.2013-ZQN-JC-2)
【分类号】：R735.7

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