大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响

发布时间：2018-07-02 09:21

本文选题：小胶质细胞 + 巨噬细胞　；参考：《第三军医大学学报》2017年11期

【摘要】：目的探讨大鼠小胶质细胞及外周血巨噬细胞对胶质瘤C6细胞株迁移能力的影响。方法将大鼠小胶质细胞及外周血单核细胞诱导分化形成的巨噬细胞分别与胶质瘤C6细胞共培养,以大鼠成纤维细胞共培养为阳性对照,单纯胶质瘤C6细胞为空白对照,共培养0、24、48 h分别行划痕实验,于划痕后6 h观察划痕实验结果;共培养24、48 h时以CD11b标记、流式分选分离小胶质/巨噬细胞及C6细胞,行Western blot检测0、24、48 h小胶质/巨噬细胞IL-10、IL-18和MMP-9及C6细胞株TGF-β、FAS配体表达水平的改变。结果早期小胶质细胞及巨噬细胞均抑制胶质瘤的迁移,巨噬细胞的抑制效果更强(P0.05);与胶质瘤细胞共培养后两种细胞对肿瘤的抑制作用消失且差异无统计学意义,均表现为促进肿瘤迁移。小胶质细胞、巨噬细胞在初始阶段IL-10、IL-18、金属基质蛋白酶MMP-9表达略有不同,但共培养后两种细胞的上述蛋白表达均升高且一致;共培养后的胶质瘤细胞的TGF-β、FAS配体较共培养前表达均升高且一致。结论小胶质细胞及巨噬细胞与C6胶质瘤细胞共培养后被驯化成同样的GAMs,对C6细胞迁移影响的生物学表现一致。
[Abstract]:Objective to investigate the effects of microglia and peripheral blood macrophages on the migration of glioma C6 cells. Methods Rat microglial cells and macrophages derived from peripheral blood monocytes were co-cultured with glioma C6 cells respectively. Rat fibroblasts were co-cultured as positive control and glioma C6 cells as blank control. The results of scratch test were observed at 6 h after scratch, and CD11b labeled with CD11b was used to separate microglia / macrophages and C6 cells at 48 h after co-culture, and microglia / macrophages and C6 cells were separated by flow cytometry. The expression of IL-18 and MMP-9 in microglia / macrophages and the expression of TGF- 尾 -FAS ligand in C6 cell line were detected by Western blot. Results both microglia and macrophages inhibited the migration of gliomas in the early stage, and the inhibitory effect of macrophages was stronger (P0.05), but the inhibitory effects of the two kinds of cells on glioma disappeared after co-culture with glioma cells, and there was no significant difference between the two kinds of cells. All of them were found to promote tumor migration. The expression of IL-10 IL-18 and MMP-9 was slightly different in microglia and macrophage at the initial stage, but the expression of these proteins was increased and consistent after co-culture. The expression of TGF- 尾 -FAS ligand in co-cultured glioma cells was higher and consistent than that before co-culture. Conclusion microglia and macrophages co-cultured with C6 glioma cells were acclimated into the same GAMs.The biological effects on the migration of C6 cells were consistent.
【作者单位】：第三军医大学大坪医院野战外科研究所神经外科;
【基金】：国家自然科学基金(81172413)~~
【分类号】：R739.41

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