四溴苯三唑对宫颈癌HeLa细胞凋亡的诱导作用及其机制

发布时间：2018-07-05 00:20

  本文选题：四溴苯三唑 + 宫颈肿瘤HeLa细胞　； 参考：《吉林大学学报(医学版)》2017年03期

【摘要】：目的:探讨四溴苯三唑(TBB)对人宫颈癌HeLa细胞的促凋亡作用,阐明其对相关信号转导通路的作用机制。方法:选取处于对数生长期的人宫颈癌HeLa细胞,将其分为对照组(未给予TBB)和实验组(给予TBB)。采用MTT比色法检测HeLa细胞存活率,采用倒置显微镜观察人宫颈癌HeLa细胞形态表现,采用AnnexinⅤ-FITC/PI双染法及流式细胞术检测HeLa细胞凋亡率,采用Western blotting法检测细胞中凋亡相关蛋白p-Akt、Akt、Bcl-2、Bax、cleaved-caspase-3和Pro-caspase-3表达水平。结果:MTT法检测,与对照组比较,实验组HeLa细胞存活率明显降低(P0.01),且呈浓度依赖性;倒置显微镜下观察,实验组HeLa细胞出现体积缩小、细胞核皱缩等细胞凋亡的形态变化;AnnexinⅤ-FITC/PI双染法和流式细胞术检测,与对照组比较,实验组各时间段(3、6、12和24h)细胞凋亡率明显升高(P0.05或P0.01);Western blotting检测,与对照组比较,实验组HeLa细胞中抗凋亡蛋白p-Akt和Bcl-2表达水平逐渐降低,促凋亡蛋白Bax和cleaved-caspase-3表达水平逐渐升高,Pro-caspase-3表达水平逐渐降低(P0.05或P0.01)。结论:TBB可能通过抑制Akt信号通路的活性进而有效促进HeLa细胞凋亡。
[Abstract]:Aim: to investigate the apoptotic effect of tetrabromotriazole (TBB) on human cervical cancer HeLa cells and to elucidate the mechanism of TBB on signal transduction pathway. Methods: human cervical cancer HeLa cells in logarithmic phase were divided into control group (without TBB) and experimental group (TBB). The survival rate of HeLa cells was detected by MTT colorimetry, the morphology of human cervical cancer HeLa cells was observed by inverted microscope, the apoptosis rate of HeLa cells was detected by Annexin 鈪，

本文编号：2098098