非整倍体与肿瘤细胞致瘤性关系的初步研究

发布时间：2018-07-09 16:03

本文选题：非整倍体 + 致瘤致瘤性　；参考：《中国癌症杂志》2017年02期

【摘要】：背景与目的:非整倍体与肿瘤形成有关,本研究旨在探讨非整倍体与肿瘤细胞致瘤性的关系。方法:C3和C5细胞系是来自于同一淋巴瘤细胞系的两个亚克隆,采用CCK-8检测2个淋巴瘤细胞系C3和C5的增殖能力;应用常规染色体分析法检测这些细胞系的核型;采用软琼脂克隆形成实验检测这些细胞系的体外致瘤能力;采用Transwell小室检测这些细胞系的侵袭转移能力;通过小鼠体内成瘤实验检测这些细胞系的体内致瘤能力。结果:两个细胞系中C3增殖能力强于C5,差异有统计学意义。核型分析结果表明,这两个细胞系都是非整倍体核型,细胞系C3的众数范围是38~78,C5的众数范围是28~50,C3细胞系中亚二倍体、二倍体和超二倍体所占比例分别为20.33%、8.47%和71.2%,C5细胞系中亚二倍体、二倍体和超二倍体所占比例分别为11.11%、86.42%和2.47%。C3和C5细胞系的非整倍体细胞比例分别为95.73%和13.58%。C3细胞系可以在软琼脂中形成克隆,而C5细胞系未能形成克隆。在体外,C3细胞系的侵袭转移能力强于C5细胞系。体内致瘤实验结果表明,C3细胞系恶性程度较高,体内致瘤能力明显强于C5细胞系,C3细胞系在体内的转移灶较多,在肝脏和肾脏均有转移灶点,而C5细胞系只在肾脏出现转移灶点。结论:非整倍体与肿瘤细胞致瘤性之间存在相关性,非整倍体对细胞的恶性转化及肿瘤发生具有重要贡献。
[Abstract]:Background & AIM: aneuploidy is associated with tumor formation. This study was designed to investigate the relationship between aneuploidy and tumorigenicity of tumor cells. Methods the cell lines of C _ 3 and C _ 5 were two subclones from the same lymphoma cell line. The proliferative ability of C _ 3 and C _ 5 cell lines were detected by CCK-8, and the karyotype of these cell lines were detected by routine chromosome analysis. The in vitro tumorigenicity of these cell lines was detected by soft Agar clone formation assay, the invasion and metastasis ability of these cell lines was detected by Transwell chamber, and the tumorigenic ability of these cell lines in vivo was detected by tumorigenic assay in mice. Results: the proliferative ability of C 3 was stronger than that of C 5 in two cell lines, and the difference was statistically significant. The results of karyotype analysis showed that the two cell lines were aneuploidy, and the mode range of cell line C _ 3 was 3878C _ 5. The percentage of diploid and hyperdiploid were 20.338.47% and 71.2% respectively. The percentage of aneuploidy cells in C _ 3 and C _ 5 cell lines were 95.73% and 13.58%, respectively, which were 11.116.42% and 2.47%, respectively. The invasion and metastasis ability of C _ 3 cell line was stronger than that of C _ 5 cell line in vitro. The results of in vivo tumorigenesis test showed that the tumor inducing ability of C5 cell line was higher than that of C5 cell line, and there were more metastatic foci in vivo, and there were metastasis sites in liver and kidney. However, C5 cell line only showed metastasis site in kidney. Conclusion: there is a correlation between aneuploidy and tumorigenicity of tumor cells. Aneuploidy plays an important role in malignant transformation and tumorigenesis.
【作者单位】：上海市医药学校;
【分类号】：R730.2

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