蜂胶中CAPE对HGF诱导的HepG2细胞侵袭和迁移能力的抑制作用

发布时间：2018-07-14 19:09

【摘要】：目的:探讨咖啡酸苯乙酯(phenethyl caffeate,CAPE)对肝细胞生长因子(hepatocyte growth factor,HGF)诱导的人肝癌细胞系(Hep G2细胞)侵袭和迁移的抑制作用及机制。方法:将实验分为对照组(HGF=0 ng/m L;CAPE=0μmol/L)、诱导组(HGF=20 ng/m L;CAPE=0μmol/L)和荷药组(HGF=20 ng/m L;CAPE分别为2.5、5.0、7.5、10.0μmol/L),测定细胞黏附率,Transwell小室模拟细胞侵袭和迁移能力以及划痕实验测定细胞迁移率,蛋白免疫印迹(Western blot)法检测ELMO1、MAP4K4和FNDC3B表达。结果:与对照组比较,诱导组表现出显著的促进细胞黏附和迁移作用,荷药组中表现出对HGF诱导的细胞黏附和迁移具有显著的抑制作用(P0.05)。Transwell小室实验结果表明CAPE对HGF诱导的Hep G2细胞侵袭和迁移作用有抑制作用。Western blot结果显示,诱导组和对照组相比ELMO1和MAP4K4表达显著上调,荷药组和诱导组相比ELMO1和MAP4K4表达显著下调(P0.05);诱导组表达FNDC3B较对照组显著下降(P0.05),荷药组FNDC3B表达量较诱导组增加,但结果不显著(P0.05)。结论:CAPE能够抑制HGF诱导的Hep G2细胞的黏附、侵袭和迁移。CAPE通过上调FNDC3B和下调ELMO1、MAP4KE的表达,抑制Hep G2细胞的侵袭和迁移能力。
[Abstract]:Aim: to investigate the inhibitory effect of phenethyl caffeate (Cape) on the invasion and migration of human hepatoma cell line (Hep G2) induced by hepatocyte growth factor (hepatocyte growth). Methods: the experiment was divided into three groups: control group (HGF0 ng/m / L CAPE-0 渭 mol / L), induction group (20 ng/m / L CAPE-0 渭 mol / L) and drug bearing group (20 ng/m / L HGFGF-20 ng/m / L CAPE = 2.5 ng/m / L). The cell adhesion rate and the ability of simulating cell invasion and migration in the transwell compartment were measured by scratch assay. The expression of ELMO1 map4K4 and FNDC3B was detected by Western blot. Results: compared with the control group, the induction group showed significant effects on promoting cell adhesion and migration. The cell adhesion and migration induced by HGF-induced cell adhesion and migration were significantly inhibited in the drug group (P0.05). The results of Transwell chamber experiment showed that Cape could inhibit the invasion and migration of HepG2 cells induced by HGF. Western blot showed that Cape could inhibit the invasion and migration of HGF-induced Hep G2 cells. The expression of ELMO1 and MAP4K4 was significantly up-regulated in the induced group compared with the control group, the expression of ELMO1 and MAP4K4 was down-regulated significantly (P0.05), the expression of FNDC3B in the induced group was significantly lower than that in the control group (P0.05), the expression of FNDC3B in the drug group was higher than that in the induced group, but the results were not significant (P0.05). Conclusion:% Cape can inhibit the adhesion, invasion and migration of HGF induced Hep G2 cells. Cape can inhibit the invasion and migration of Hep G2 cells by upregulating FNDC3B and down-regulating the expression of ELMO1 MAP4KE.
【作者单位】： 南京农业大学食品科技学院;中国农业科学院蜜蜂研究所;
【基金】：中国农业科学院科技创新工程专项(CAAS-ASTIP-2015-IAR)
【分类号】：TS201.4;R735.7

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