ARF在胃癌组织中的表达及临床意义

发布时间：2018-07-15 21:33

【摘要】：目的:初步探讨ARF(ADP-核糖基化因子)在胃癌组织中的表达及其临床意义。方法:选取2013年12月至2014年6月经手术切除并病理确诊的原发性胃癌组织标本及癌旁组织标本各50例。应用免疫组化检测ARF在胃癌和癌旁组织中蛋白的表达,初步探讨其在胃癌及癌旁及组织中的蛋白表达水平差异及与胃癌患者临床病理参数的相关性。应用实时荧光定量PCR(RT-q PCR,Real-Time quantitative polymerase chain reaction)方法检测胃癌组织及癌旁组织中ARF m RNA的表达,初步探讨其在胃癌组织及癌旁组织中的m RNA表达差异情况。结果:ARF在胃癌组织中的蛋白阳性表达率为72%(36/50),显著高于癌旁组织表达10%(5/50)(P0.05),且胃癌组织中的累积光密度值(IOD27707.02±17815.72)与癌旁组织中的累积光密度值(IOD12704.16±13932.79)比较有极显著差异,ARF蛋白表达与胃癌患者的性别、年龄、部位、肿瘤大小及分化程度无明显相关,(P0.05),与胃癌浸润深度、淋巴结转移及TNM分期有关(P0.05);胃癌组织中ARF的m RNA表达较癌旁组织升高(P0.05)。结论:(1)胃癌组织中ARF蛋白及m RNA水平的表达明显高于癌旁组织,提示ARF蛋白及基因的检测可能作为检测胃癌有用的生物学标志之一。(2)ARF蛋白表达与胃癌患者的性别、年龄、部位、肿瘤大小及分化程度无关,与胃癌浆膜有无浸润、淋巴结转移及TNM分期有关,其可能在胃癌发生、发展中起重要作用,有可能成为判断胃癌患者预后的标志之一。
[Abstract]:Objective: to investigate the expression and clinical significance of ARF in gastric carcinoma. Methods: from December 2013 to June 2014, 50 specimens of primary gastric cancer and 50 specimens of paracancerous tissues were selected. Immunohistochemistry was used to detect the expression of ARF in gastric cancer and paracancerous tissues. The expression level of ARF in gastric cancer and adjacent tissues was preliminarily studied and the correlation between ARF and clinicopathological parameters of gastric cancer was investigated. The expression of ARF mRNA in gastric cancer tissues and adjacent tissues was detected by RT-q PCR Real-Time quantitative polymerase chain reaction) method, and the difference of ARF mRNA expression in gastric cancer tissues and paracancerous tissues was preliminarily investigated. Results the positive rate of protein expression in gastric cancer was 72% (36 / 50), which was significantly higher than that in paracancerous tissues (10%, 5 / 50) (P0.05). The cumulative optical density (IOD27707.02 卤17815.72) in gastric cancer tissues was significantly different from that in adjacent tissues (IOD12704.16 卤13932.79). To the gender of patients with gastric cancer, Age, location, tumor size and differentiation were not significantly related to the depth of gastric cancer invasion, lymph node metastasis and TNM staging (P0.05); the expression of ARF mRNA in gastric cancer tissue was higher than that in adjacent tissues (P0.05). Conclusion: (1) the expression of ARF protein and mRNA in gastric cancer tissues is significantly higher than that in adjacent tissues, suggesting that the detection of ARF protein and gene may be one of the useful biological markers for the detection of gastric cancer. (2) the expression of ARF protein and the sex, age and location of gastric cancer patients. Tumor size and differentiation are not related to serous infiltration, lymph node metastasis and TNM staging of gastric cancer, which may play an important role in the occurrence and development of gastric cancer, and may be one of the markers to judge the prognosis of gastric cancer patients.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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