非小细胞肺癌患者血清miR-22和miR-126的检测及其临床意义
发布时间：2018-07-16 20:31
【摘要】：目的:探讨液体活检外周血清miR-22和miR-126的表达在非小细胞肺癌(NSCLC)发生和转移中的作用。方法:收集盐城市第六人民医院2013年5月至2015年5月期间确诊的127例NSCLC患者,设健康对照112例,采用q PCR检测患者血清中miR-22和miR-126的相对表达水平,Logistic回归分析法及ROC曲线分析miR-22和miR-126在NSCLC发展与转移预测中的敏感性及特异性。结果:病例组血清miR-22水平显著高于对照组,而血清miR-126水平低于对照组(均P0.05);与腺癌患者相比,鳞状细胞癌患者的血清miR-22水平显著升高(P0.05)、血清miR-126显著降低(P0.01);与Ⅰ+Ⅱ期患者相比,Ⅲ+Ⅳ期患者血清miR-22水平升高、miR-126水平相对降低(均P0.01);与未转移患者相比,转移患者血清miR-22水平升高,而血清miR-126水平降低(均P0.01);有家族遗传史的患者与没有家族遗传史的患者相比,血清miR-22水平升高但miR-126水平降低(均P0.01)。血清miR-22与miR-126水平对NSCLC预测的特异性与敏感性分别为99.11%和84.30%、82.68%和96.40%。血清miR-22与miR-126水平对NSCLC转移的特异性与敏感性分别为59.74%和96.00%、84.00%和62.30%。结论:在NSCLC患者血清中,miR-22呈高表达而miR-26呈低表达,液体活检miR-22和miR-126可作为预测NSCLC的发生及转移的一个生物学指标。
[Abstract]:Aim: to investigate the role of miR-22 and miR-126 in the pathogenesis and metastasis of non-small cell lung cancer (NSCLC). Methods: 127 patients with NSCLC were collected from the sixth people's Hospital of Yancheng from May 2013 to May 2015. The relative expression level of miR-22 and miR-126 in serum was detected by qPCR and the sensitivity and specificity of miR-22 and miR-126 in the prediction of NSCLC development and metastasis were analyzed by logistic regression analysis and ROC curve analysis. Results: the serum miR-22 level in the case group was significantly higher than that in the control group, while the serum miR-126 level was lower than that in the control group (P0.05), the serum miR-22 level in squamous cell carcinoma patients was significantly higher than that in adenocarcinoma patients (P0.05), the serum miR-126 level was significantly lower (P0.01), and the level of miR-126 in patients with stage 鈪，
本文编号：2127589
[Abstract]:Aim: to investigate the role of miR-22 and miR-126 in the pathogenesis and metastasis of non-small cell lung cancer (NSCLC). Methods: 127 patients with NSCLC were collected from the sixth people's Hospital of Yancheng from May 2013 to May 2015. The relative expression level of miR-22 and miR-126 in serum was detected by qPCR and the sensitivity and specificity of miR-22 and miR-126 in the prediction of NSCLC development and metastasis were analyzed by logistic regression analysis and ROC curve analysis. Results: the serum miR-22 level in the case group was significantly higher than that in the control group, while the serum miR-126 level was lower than that in the control group (P0.05), the serum miR-22 level in squamous cell carcinoma patients was significantly higher than that in adenocarcinoma patients (P0.05), the serum miR-126 level was significantly lower (P0.01), and the level of miR-126 in patients with stage 鈪，
本文编号：2127589
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